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Nucleic Acids Research, 1993, Vol. 21, No. 3 413-417
© 1993

MOLECULAR BIOLOGY

Pleiotrophic effects of point mutations in yeast tRNAAsp on the base modification pattern

Johan Edqvist, Kerstin B. Sträby and Henri Grosjean1

Department of Microbiology, University of Umeå S-901 87 Umeå, Sweden 1Laboratoire d'Enzymologie, CNRS F-91198 Gif-sur-Yvette, France

To whom correspondence should be addressed

Received November 17, 1992. Accepted January 11, 1993.

The base-modification pattern has been studied in several synthetic variants of yeast tRNAAsp injected into Xenopus laevis oocytes. Certain point mutations in the D-stem and the varlabie loop of the tRNA led to considerabiy decreased levels of m1G37, {psi}40 and Q34/manQ34 in the anticodon stem or loop and an increased rate of synthesis for m5C49 in the T-stem. The formation of m2G6 in the aminoacyl-stem was not atfected in any of the tRNA-variants. Thus, mutations in one part of the tRNA-moiecuie can have long-range effects on the interactions between another part of the IRNA and the tRNA modifying enzymes.


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