Nucleic Acids Research, 1993, Vol. 21, No. 3 547-553
© 1993
MOLECULAR BIOLOGY |
Isolation of a cDNA encoding the adenovirus E1A enhancer binding protein: a new human member of the ets oncogene family
Department of Molecular Biology, Cancer Research Institute, Sapporo Medical College S-i, W-i 7, Chuo-ku, Sapporo 060, Japan
*To whom correspondence should be addressed
Received October 19, 1992. Revised January 11, 1992. Accepted January 11, 1993.
The cDNA encoding adenovirus E1A enhancer-binding protein E1A-F was isolated by screening a HeLa cell 
gt11 expression library for E1A-F site-specific DNA binding. One cDNA clone produced recombinant E1A- F protein with the same DNA binding specificity as that endogenous to HeLa cells. Sequence analysis of the CDNA showed homology with the ETS-domain, a region required for sequence-specific DNA binding and common to all ets oncogene members. Analysis of the longest cDNA revealed about a 94% identity in amino acids between human E1A-F and mouse PEA3 (poiyomavirus enhancer activator 3), a recently characterized ets oncogene member. E1A-F was encoded by a 2.5kb mRNA in HeLa cells, which was found to increase during the early period of adenovirus infection. In contrast, ets-2 mRNA was significantly reduced in infected HeLa cells. The results indicate that E1A enhancer binding protein E1A-F is a member of the ets oncogene family and is probably a human homologue of mouse PEA3.
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