Skip Navigation

This Article
Right arrow Print PDF (609K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zhang, B.
Right arrow Articles by Blumenthal, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zhang, B.
Right arrow Articles by Blumenthal, R. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 4 905-911
© 1993

MOLECULAR BIOLOGY

The M·AluI DNA-(cytosine C5)-methyltransferase has an unusually large, partially dispensable, variable region

Bohong Zhang, Tao Tao1, Geoffery G. Wilson and Robert M. Blumenthal1,*

New England BioLabs Inc., Beverly, MA 01915-5599 1Department of Microbiology, Medical College of Ohio Toledo, OH 43699-0008, USA

* To whom correspondence should be addressed

Received November 16, 1992. Revised January 9, 1993. Accepted January 9, 1993.

The DNA methyltransferase of the AluI restriction-modification system, from Arthrobacter luteus, converts cytosine to 5-methylcytosine in the sequence AGCT. The gene for this methyltransferase, aluIM, was cloned into Escherichla coli and sequenced. A 525-codon open reading frame was found, consistent with deletion evidence, and the deduced amino acid sequence revealed all ten conserved regions common to 5-methylcytosine methyltransferases. The aluIM sequence predicts a protein of Mr 59.0k, in agreement with the observed Mr making M·AluI the largest known methyltransferase from a type II restriction-modification system. M·AluI also contains the largest known variable region of any monospecific DNA methyltransferase, larger than that of most multispecific methyltransferases. In other DNA methyltransferases the variable region has been implicated as the sequence-specific target recognition domain. An in-frame deletion that removes a third of this putative target-recognition region leaves the AluI methyltransferase still fully active.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 MicrobiologyHome page
R. M. Dempsey, D. Carroll, H. Kong, L. Higgins, C. T. Keane, and D. C. Coleman
Sau42I, a BcgI-like restriction-modification system encoded by the Staphylococcus aureus quadruple-converting phage {pi}42
Microbiology, April 1, 2005; 151(4): 1301 - 1311.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Laging, B. Fartmann, and W. Kramer
Isolation of segments of homologous genes with only one conserved amino acid region via PCR
Nucleic Acids Res., January 15, 2001; 29(2): e8 - e8.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.