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Nucleic Acids Research, 1993, Vol. 21, No. 4 977-985
© 1993

MOLECULAR BIOLOGY

IGH minisatellite suppression of USF-binding-site- and Eµ-mediated transcriptional activation of the adenovirus major late promoter

William L. Trepicchio+ and Theodore G. Krontiris*

Department of Medicine (Hematology/Oncology), New England Medical Center Hospitals, Graduate Program in Immunology, Tufts Universrty Schcml of Medicine 750 Washington Street, Boston, MA 021 11, USA

*To whom correspondence should be addressed

Received October 16, 1992. Revised December 18, 1992. Accepted December 18, 1992.

The 50bp repeat unit of the minisatellite within the DH JH interval of the human immunoglobulin heavy chain locus binds a nuclear factor present in a wide variety of cell types. The binding site contains the myc/HLH motif, CACGTG, and represents a 15 of 17 base match for the USF/WLTF binding site adjacent to the adenovirus major late promoter (MLP). Unlike the USF/MLTF site, the IGH minisatellite possesses no enhancer activity. However, it can significantly suppress, in cis and in trans, USF-site-mediated transcriptional activation of the MLP. In murine myeloma cells, the IGH minisatellite can suppress, in trans, MLP activation by the murine heavy chain gene enhancer, Eµ. These activities potentially represent a DNA-based form of squelching.

+Present address: The Biological Laboratories, Harvard University, Cambridge, MA, USA


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