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Nucleic Acids Research, 1993, Vol. 21, No. 6 1345-1349
© 1993

MOLECULAR BIOLOGY

Evolutionary conservation of excision repair in Schizosaccharomyces pombe: evidence for a family of sequences related to the Saccharomyces cerevisiae RAD2 gene

Antony M. Carr, Katherine S. Sheldrick, S. Sheldrick, Johanne M. Murray1, Rowyda Al-Harithy1, Felicity Z. Watts1 and Alan R. Lehmann

MRC Cell Mutation Unit, Sussex University, Falmer, Brighton BN1 9RR Falmer, Brighton BN1 9QG, UK 1School of Biological Sciences, Sussex University Falmer, Brighton BN1 9QG, UK

Received January 15, 1993. Revised February 16, 1993. Accepted February 16, 1993.

Cells mutated at the rad13 locus In the fission yeast, Schizosaccharomyces pombe are deficient In excision-repair of UV damage. We have cloned the S.pombe rad13 gene by its ability to complement the UV sensitivity of a rad13 mutant. The gene Is not essential for cell proliferation. Sequence analysis of the cloned gene revealed an open reading-frame of 1113 amino acids with structural homology to the RAD2 gene of the distantly related Saccharomyces cerevlslae. The sequence similarity is confined to three domains, two close to the N-terminus of the encoded protein, the third being close to the C-terminus. The central region of about 500 amino acids shows little similarity between the two organisms. The first and third domains are also found in a related yet distinct pair of homologous S.pombe/S.cerevisiae DNA repair genes (rad2/YKL510), which have only a very short region between these two conserved domains. Using the polymerase chain reaction with degenerate primers, we have isolated fragments from a gene homologous to rad13/RAD2 from Aspergillus nidulans. These findings define new functional domains involved In excision-repair, as well as identifying a conserved family of genes related to RAD2.


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