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Nucleic Acids Research, 1993, Vol. 21, No. 6 1427-1431
© 1993

GENOME STRUCTURE AND MAPPING

Characterization of (GT)n and (CT)n microsatellites in two insect species: Apis mellifera and Bombus terrestris

Arnaud Estoup, Michel Solignac, Myriam Harry and Jean-Marie Cornuet1

Laboratoire de Biologie et Génétique Evolutives, CNRS Avenue de la Terrasse, 91198 Gif-sur-Yvette, France 1Laboratoire de Neurobiologie Comparée des Invértebrés INRA-CNRS URA 1190, La Guyonnerie, BP 23, 91440 Bures-sur-Yvette, France

Received December 22, 1992. Revised February 16, 1993. Accepted February 16, 1993.

A set of 52 (CT)n and 23 (GT)n microsatellltes In honeybee, 24 (CT)n and 2 (GT)n microsatellites in bumblebee (n>6) have been isolated from partial genomlc librairies and sequenced. On average, (CT)n and (GT)n microsatellites occur every 15 kb and 34 kb in honeybee and every 40 kb and 500 kb In bumble-bee, respectively. The prevailing categories are imperfect repeats for (CT)n microsatellites in bumble-bee, and perfect repeats for both (CT)n and (GT)n microsatellites in honey-bee. Comparisons with data available In vertebrates indicate a lower proportion of perfect repeats In bees but length distributions are very similar regardless the phylum. This result extends to insects the concept of an evolutionary conservation for quantitative and qualitative characteristics of (CT)n and (GT)n microsatellites. Many (CT)n and (GT)n repeats are surrounded with various types of microsatellltes, revealing an associative distribution of short repeat sequences. As expected, a high level of Intrapopulatlonal polymorphism has been found with one tested honeybee mlcrosatellite. Also, flanking regions of this microsatellite are similar enough to allow PCR amplification in several other species of Apis and Bombus.


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