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Nucleic Acids Research, 1993, Vol. 21, No. 6 1449-1455
© 1993

MOLECULAR BIOLOGY

The sequence of 28S ribosomal RNA varies within and between human cell lines

Henrik Leffers and Annette H. Andersen

Institute ot Medical Biochemistry and Danish Centre for Human Genome Research, Ole Worms Allé, Building 170, University Park Aarhus University, DK-8000 Aarhus C, Denmark

Received December 1, 1992. Revised February 22, 1993. Accepted February 22, 1993.

The primary structure of 28S ribosomal RNA constitutes a conserved core which Is similar among most 23S-llke rRNAs and expansion segments which occur at specific positions in the sequence. The expansion segments account for most of the size difference between prokaryotic (archaeal and eubacterlal) and eukaryotlc rRNAs and they exhibit a sequence variation which Is unique among rRNAs. We have Investigated the sequence variation of one of the expansion segments, V8, by sequencing a total of 111 V8 segments from 9 different human cell lines and tissues and have found 35 different variants. The variation occur mainly at two ‘hot spots’ which are separated by 170 nucleotldes In the primary sequence but are neighbours in the secondary structure. The sequence of V8 segments varies both within and between human cell lines and tissues. The Implications for the evolution of the eukaryotlc 28S rRNA are discussed together with possible functions of the expansion segments. We also present a secondary structure model for the V8 segment based on comparative sequence analysis and chemical and enzymatic foot printing.


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