Skip Navigation

This Article
Right arrow Print PDF (5213K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pochart, P.
Right arrow Articles by Heyman, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pochart, P.
Right arrow Articles by Heyman, T.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 7 1517-1521
© 1993

MOLECULAR BIOLOGY

A very poorly expressed tRNASer is highly concentrated together with replication primer initiator tRNAMet in the yeast Ty1 virus-like particles

P. Pochart, B. Agoutin, C. Fix1, G. Keith1,* and T. Heyman

Institut Curie-Biologie, Centre Universitaire 91405, Orsay, France 1Unité ‘Structure des Macromolécules Biologiques et Mécanismes de Reconnaissance’, Institut de Biologie Moléculaire et Cellulaire du CNRS et Université Louis Pasteur 67084 Strasbourg, France

* To whom correspondence should be addressed

Received March 1, 1993. Accepted March 8, 1993.

The analysis of the tRNAs associated to the virus-like particles produced by the Ty1 element revealed the specific packaging of three major tRNA species, in about equal amounts: the replication primer initiator tRNAMet, the tRNASerAGA and a tRNA undetected until now as an expressed species in yeast. The latter tRNA is coded by the already described tDNASerGCT. This tRNA is enriched more than 150 fold in the particles as compared to its content in total cellular tRNA where it represents less than 0.1% (initiator tRNAMet and tRNASerAGA being 11 and 4 fold enriched respectively). This tRNA is the only species coded by the tDNASerGCT gene which is found in three copies per genome since no other corresponding expressed tRNA could be detected. This gene is thus very poorly expressed. The high concentration of tRNASerGCU in the particles compared to its very low cellular content led us to consider its possible implication in Ty specific processes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
E. H. Mules, O. Uzun, and A. Gabriel
In Vivo Ty1 Reverse Transcription Can Generate Replication Intermediates with Untidy Ends
J. Virol., August 1, 1998; 72(8): 6490 - 6503.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
S. Friant, T. Heyman, A. S. Byström, M. Wilhelm, and F. X. Wilhelm
Interactions between Ty1 Retrotransposon RNA and the T and D Regions of the tRNAiMet Primer Are Required for Initiation of Reverse Transcription In Vivo
Mol. Cell. Biol., February 1, 1998; 18(2): 799 - 806.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.