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Nucleic Acids Research, 1993, Vol. 21, No. 7 1629-1635
© 1993

MOLECULAR BIOLOGY

Estrogen-inducible and liver-specific expression of the chicken Very Low Density Apolipoprotein II gene locus in transgenic mice

Jan Wijnholds, Sjaak Philipsen1, Sara Pruzina1, Peter Fraser1, Frank Grosveld1 and Geert AB*

Laboratory of Biochemistry, Groningen University Nijenborgh 4, 9747 AG Groningen, The Netherlands 1Laboratory of Gene Structure and Expression, National Institute for Medical Research The Ridgeway, Mill Hill, London NW7 1AA, UK

* To whom correspondence should be addressed

Received December 1, 1993. Revised March 4, 1993. Accepted March 4, 1993.

We have examined the chicken Very Low Density Apolipoprotein II (apoVLDL II) gene locus in transgenic mice. A DNA fragment composed of the transcribed region, 16 kb of 5' flanking and 400 bp of 3' flanking sequences contained all the information sufficient for estrogen-inducible, liver-specific expression of the apoVLDL II gene. The far-upstream region contains a Negative Regulating Element coinciding with a DNaseihypersensitive site at –11 kb. in transgenic mice, the NRE at –11 kb is used for downregulating the expression to a lower maximum level. The NRE might be used for modulating apoVLDL II gene expression, and may be involved in the rapid shut-down of the expression after hormone removal.


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