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Nucleic Acids Research, 1993, Vol. 21, No. 8 1761-1766
© 1993

MOLECULAR BIOLOGY

Isolation of cDNA encoding a binding protein specific to 5'-phosphorylated single-stranded DNA with G-rich sequences

Tatsunobu-Ryushin Mizuta1,+, Yosho Fukita2, Takashi Miyoshi2, Akira Shimizu1 and Tasuku Honjo1,2,*

1Center for Molecular Biology and Genetics, Kyoto University Sakyo-ku, Kyoto 606-01, Japan 2Department of Medical Chemistry, Kyoto University Faculty of Medicine Sakyo-ku, Kyoto 606-01, Japan

* To whom correspondence should be addressed

Received January 29, 1993. Revised March 17, 1993. Accepted March 17, 1993.

We have isolated the cDNA encoding a binding protein to the sequence motif of the Immunoglobulin Sµ region by the southwestern method. The binding protein designated Sµbp-2 specifically binds to 5'-phos-phorylated single-stranded DNA containing 5'-G and GGGG stretches. The amlno acid sequence deduced from the the cDNA sequence showed that the Sµbp-2 belongs to the putative hellcase superfamlly which is involved in replication, recombination and repair. Expression of Sµbp-2 mRNA is ubiquitous and augmented In spleen cells stimulated with llpopolysaccharide and interteukln 4 which also Induce class switching. The Sµbp-2 gene Is conserved among vertebrates. Possible Involvement of Sµbp-2 in class switching is discussed.

+ Present Address: Howard Hughes Medical Institute Research Laboratories, The Children's Hospital, Boston, MA 02115, USA


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