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Nucleic Acids Research, 1993, Vol. 21, No. 8 1775-1781
© 1993

MOLECULAR BIOLOGY

A repetitive DNA sequence associated with the centromeres of Chironomus pallidivittatus

Carlos Rovira, Wolfgang Beermann1 and Jan-Erik Edström*

University of Lund, Department of Molecular Genetics Solvegatan 29, S-223 62 LUND, Sweden 1Eduard Spranger-Strasse 55, D-7400 Tubingen 1, Germany

* To whom correspondence should be addressed

Received January 1, 1993. Revised March 19, 1993. Accepted March 19, 1993.

A clone containing centromere-associated DNA from Chlronomus pallldlvlttatus was obtained by mlcrodissectlon-microcloning. It hybridizes to the centromeric end of one chromosome and exclusively to regions In the three remaining, metacentrlc chromosomes to which centromeres have previously been localized on cytologlcal grounds. In the metacentrlc positions the hybridization can be assigned to thin bands. The clone contains 155bp tandem repeats and short flanking regions represented In all of the centromeres. Titratlon experiments show that the four centromeres together contain 200kb of 155bp repeat per genome. In a line of tissue culture cells the amounts are increased by a factor 1.5–2, resulting In proportionately extended arrays of tandem repeats. Each repeat contains two Invertrepeats surrounding a region containing only AT base pairs, a feature with some similarity to functionally essential elements in the Saccharomyces cerevlslae centromere.


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