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Nucleic Acids Research, 1993, Vol. 21, No. 8 1805-1809
© 1993

MOLECULAR BIOLOGY

Molecular characterization of the Salmonella typhimurium parE gene

Amy L. Springer+ and Molly B. Schimd*

Department of Molecular Biology, Princeton University Princeton, NJ 08544, USA

* To whom correspondence should be addressed

Received January 21, 1993. Revised March 19, 1993. Accepted March 19, 1993.

The DNA sequence of the wild type S. typhlmurium parE gene was determined. The predicted protein has 96.7% amlno acid Identity with the ParE protein of E.coll, but is 29 amlno adds longer, due to an additional basepair In the 3' end of the S. typhlmurium gene. Subclones of the S. typhlmurium parE gene localized the sites of four heat sensitive mutations within parE. The parE206 and parE374 mutations are Identical (Val67-Met) and lie In a highly conserved region corresponding to the ATP binding pocket of GyrB. Two additional heat sensitive mutations were sequenced and predict the following amino acid substitutions: parE377 (Gly399-Ser) and parE493 (Thr583-Pro). All of the heat sensitive mutations Me In regions with strong amlno acid homology to GyrB.

+Present address: Department of Environmental Engineering, W.M. Keck Laboratories, 138–78 California Institute of Technology, CA, USA


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