Skip Navigation

This Article
Right arrow Print PDF (2194K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (81)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Baubonis, W.
Right arrow Articles by Sauer, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baubonis, W.
Right arrow Articles by Sauer, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1993, Vol. 21, No. 9 2025-2029
© 1993

MOLECULAR BIOLOGY

Genomic targeting with purified Cre recombinase

Wendy Baubonis1 and Brian Sauer*,1

1Biotechnology R & D, DuPont Merck Pharmaceutical Co. Experimental Station E400, Wilmington, DE 19880-0400, USA

* To whom correspondence should be addressed

Received March 23, 1993. Accepted February 26, 1993.

Purified Cre recombinase protein Introduced directly Into cultured mammalian cells by lipofection catalyzes both site-specific chromosomal Integration of a cotransfected lox targeting vector and precise excision of genomic DNA flanked by directly repeated lox sites. This procedure ellminates the need to transfect cre expression piasmids to activate recombination at lox sites. We used this simplified procedure to investigate the effect on targeting efficiency of both lox vector design and chromosomal position of the lox target. We show that such chromosomal position effects can exert at least a 50-fold per lox target difference in targeting efficiency in a human osteosarcoma cell line.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
S. De Buck, I. Peck, C. De Wilde, G. Marjanac, J. Nolf, A. De Paepe, and A. Depicker
Generation of Single-Copy T-DNA Transformants in Arabidopsis by the CRE/loxP Recombination-Mediated Resolution System
Plant Physiology, December 1, 2007; 145(4): 1171 - 1182.
[Abstract] [Full Text] [PDF]

Home page
 Circ. Res.Home page
D. E. Gutstein, G. E. Morley, H. Tamaddon, D. Vaidya, M. D. Schneider, J. Chen, K. R. Chien, H. Stuhlmann, and G. I. Fishman
Conduction Slowing and Sudden Arrhythmic Death in Mice With Cardiac-Restricted Inactivation of Connexin43
Circ. Res., February 16, 2001; 88(3): 333 - 339.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
A. Moon, A. Boulet, and M. Capecchi
Normal limb development in conditional mutants of Fgf4
Development, January 3, 2000; 127(5): 989 - 996.
[Abstract] [PDF]

Home page
 J. Virol.Home page
S. Lamartina, G. Roscilli, D. Rinaudo, P. Delmastro, and C. Toniatti
Lipofection of Purified Adeno-Associated Virus Rep68 Protein: toward a Chromosome-Targeting Nonviral Particle
J. Virol., September 1, 1998; 72(9): 7653 - 7658.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
U. Ahlgren, J. Jonsson, L. Jonsson, K. Simu, and H. Edlund
beta -Cell-specific inactivation of the mouse Ipf1/Pdx1 gene results in loss of the beta -cell phenotype and maturity onset diabetes
Genes & Dev., June 15, 1998; 12(12): 1763 - 1768.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
G Giuili, W. Shen, and H. Ingraham
The nuclear receptor SF-1 mediates sexually dimorphic expression of Mullerian Inhibiting Substance, in vivo
Development, January 5, 1997; 124(9): 1799 - 1807.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.