Nucleic Acids Research, 1993, Vol. 21, No. 9 2229-2235
© 1993
MOLECULAR BIOLOGY |
The Rb97D gene encodes a potential RNA-binding protein required for spermatogenesis in Drosophila
Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda MD 20892, USA
* To wbonrcorrespondence should be addressed
Received September 8, 1992. Revised March 29, 1993. Accepted March 29, 1993.
Many proteins that bind RNA contain a common RNA-blndlng domain, the RNP motif. We have been studying two Drosophila RNP motif proteins, Hrb98DE and Hrb87F, which are hnRNA-blndlng proteins. We report here the characterization of the Rb97D gene, which encodes a protein that Is closely related to the Hrb proteins In the RNP motif domain, but has a distinctive prollne-rlch C-termlnal domain. The gene is located at 97D on the right arm of the third chromosome, near the rough gene. Multiple transcripts from the Rb97D gene are present at varying levels throughout development. The transcripts are generated by alternative processing In the coding and 3’ untranslated regions, and can encode two protein Isoforms. Analysis of a mutant containing a P element inserted Into the 5’ untranslated region of the gene demonstrates that Rb97D is required for male fertility. Possible models for the function of Rb97D in testes are discussed.
+ Present address: Laboratory of Biochemistry, National Cancer Institute, National Institute of Health, Bethesda, MD 20892, USA
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