Nucleic Acids Research, 1994, Vol. 22, No. 1 59-65
© 1994
MOLECULAR BIOLOGY |
Transcriptional repression by the human bZIP factor E4BP4: definition of a minimal repression domain
Department of Biochemistry and Genetics, The Medical School, University of Newcastle Upon Tyne Newcastle Upon Tyne NE2 4HH , UK 1Imperial Cancer Research Fund Oncology Group, Hammersmith Hospital Du Cane Road, London W12 OHS, UK
Received October 1, 1993. Accepted December 8, 1993.
The bZIP factor E4BP4 overlaps in DNA binding site specificity with the transcriptional activator CREB and members of the ATF family of transcription factors, but is an active transcriptional repressor. In this study we have mapped the repressing activity of E4BP4 to a small ‘domain’ of 65 amino acids that retains its ability to repress transcription when transferred to the heterologous DNA binding domain of the yeast transcriptional activator GAL4. This segment of the E4BP4 polypeptide contains a high proportion of charged amino acids and does not resemble the repression domains that have been characterized so far from other active transcriptional repressors such as the Drosophila Krüppel, Engralled or Even-skipped proteins. A mutation which changes the charge configuration of this repression module resulted in a complete loss of repressor activity. The E4BP4-GAL4 fusion protein is able to repress the residual transcription from minimal promoters containing the adenovirus E4 or E1 b TATA box. This is consistent with a mechanism of action whereby E4BP4 interacts with some component of the general transcription machinery to cause repression of basal and activated transcription. Although a number of nuclear proteins are able to interact with the E4BP4 repression domain in vitro, these proteins do not appear to include the general transcription factors TFIIB or TBP.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  C. Hough, C. D. Cuthbert, C. Notley, C. Brown, C. Hegadorn, E. Berber, and D. Lillicrap Cell type-specific regulation of von Willebrand factor expression by the E4BP4 transcriptional repressor Blood, February 15, 2005; 105(4): 1531 - 1539. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Stepchenko and M. Nirenberg Mapping activation and repression domains of the vnd/NK-2 homeodomain protein PNAS, September 7, 2004; 101(36): 13180 - 13185. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Szuplewski, B. Kottler, and R. Terracol The Drosophila bZIP transcription factor Vrille is involved in hair and cell growth Development, August 15, 2003; 130(16): 3651 - 3662. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C. Ozkurt and S. Tetradis Parathyroid Hormone-induced E4BP4/NFIL3 Down-regulates Transcription in Osteoblasts J. Biol. Chem., July 11, 2003; 278(29): 26803 - 26809. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-L. Yu, Y.-J. Chiang, and J. J. Y. Yen GATA Factors Are Essential for Transcription of the Survival Gene E4bp4 and the Viability Response of Interleukin-3 in Ba/F3 Hematopoietic Cells J. Biol. Chem., July 19, 2002; 277(30): 27144 - 27153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Mitsui, S. Yamaguchi, T. Matsuo, Y. Ishida, and H. Okamura Antagonistic role of E4BP4 and PAR proteins in the circadian oscillatory mechanism Genes & Dev., April 15, 2001; 15(8): 995 - 1006. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Uchida, Y. Tanaka, H. Ito, F. Saitoh-Ohara, J. Inazawa, K. K. Yokoyama, S. Sasaki, and F. Marumo Transcriptional Regulation of the CLC-K1 Promoter by myc-Associated Zinc Finger Protein and Kidney-Enriched Kruppel-Like Factor, a Novel Zinc Finger Repressor Mol. Cell. Biol., October 1, 2000; 20(19): 7319 - 7331. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. Ishida, K. Ueda, K. Ohkawa, Y. Kanazawa, A. Hosui, F. Nakanishi, E. Mita, A. Kasahara, Y. Sasaki, M. Hori, et al. Identification of Multiple Transcription Factors, HLF, FTF, and E4BP4, Controlling Hepatitis B Virus Enhancer II J. Virol., February 1, 2000; 74(3): 1241 - 1251. [Abstract] [Full Text]
|
|
|
|
|
|
C.-K. Lai and L.-P. Ting Transcriptional Repression of Human Hepatitis B Virus Genes by a bZIP Family Member, E4BP4 J. Virol., April 1, 1999; 73(4): 3197 - 3209. [Abstract] [Full Text]
|
|
|
|
|
|
R. A. Altura, T. Inukai, R. A. Ashmun, G. P. Zambetti, M. F. Roussel, and A. T. Look The Chimeric E2A-HLF Transcription Factor Abrogates p53-Induced Apoptosis in Myeloid Leukemia Cells Blood, August 15, 1998; 92(4): 1397 - 1405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Zhang and G. Childs Human ZFM1 Protein Is a Transcriptional Repressor That Interacts with the Transcription Activation Domain of Stage-specific Activator Protein J. Biol. Chem., March 20, 1998; 273(12): 6868 - 6877. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Passantino, V. Antona, G. Barbieri, P. Rubino, R. Melchionna, G. Cossu, S. Feo, and A. Giallongo Negative Regulation of beta Enolase Gene Transcription in Embryonic Muscle Is Dependent upon a Zinc Finger Factor That Binds to the G-rich Box within the Muscle-specific Enhancer J. Biol. Chem., January 2, 1998; 273(1): 484 - 494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Denver, S. Pavgi, and Y.-B. Shi Thyroid Hormone-dependent Gene Expression Program for Xenopus Neural Development J. Biol. Chem., March 28, 1997; 272(13): 8179 - 8188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Ikushima, T. Inukai, T. Inaba, S. D. Nimer, J. L. Cleveland, and A. T. Look Pivotal role for the NFIL3/E4BP4 transcription factor in interleukin 3-mediated survival of pro-B lymphocytes PNAS, March 18, 1997; 94(6): 2609 - 2614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Nishimura and T. Tanaka Calcium-dependent Activation of Nuclear Factor Regulated by Interleukin 3/Adenovirus E4 Promoter-binding Protein Gene Expression by Calcineurin/Nuclear Factor of Activated T Cells and Calcium/Calmodulin-dependent Protein Kinase Signaling J. Biol. Chem., June 1, 2001; 276(23): 19921 - 19928. [Abstract] [Full Text] [PDF]
|
|