Nucleic Acids Research, 1994, Vol. 22, No. 11 1981-1987
© 1994
MOLECULAR BIOLOGY |
Interaction between the first and last nucleotides of pre-mRNA introns is a determinant of 3' splice site selection in S.cerevisiae
Unité de Génétique Moléculaire des Levures (URA 1149 du CNRS), Département de Biologie Moléculaire Institut Pasteur, 25 rue du Dr Roux, F-75724 Paris CEDEX 15, France
*To whom correspondence should be addressed
Received April 8, 1994. Accepted May 5, 1994.
The splicing of group II and nuclear pre-mRNAs introns occurs via a similar splicing pathway and some of the RNA-RNA interactions involved in these splicing reactions show structural similarities. Recently, genetic analyses performed in a group II intron and the yeast nuclear actin gene suggested that non Watson-Crick interactions between intron boundaries are important for the second splicing step efficiency in both classes of introns. We here show that, in the yeast nuclear rp51A intron, a G to A mutation at the first position activates cryptic 3' splice sites with the sequences UAC/ or UAA/. Moreover, the natural 3' splice site could be reactivated by a G to C substitution of the last intron nucleotide. These results demonstrate that the interaction between the first and last intron nucleotides is a conserved feature of nuclear pre-mRNA splicing in yeast and is involved in the mechanism of 3' splice site selection.
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