Nucleic Acids Research, 1994, Vol. 22, No. 11 2057-2064
© 1994
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Splicing defective mutants of the COXI gene of yeast mitochondrial DNA: initial definition of the maturase domain of the group II intron AI2
Department of Biochemistry, University of Texas Southwestern Medical Center Dallas, TX 75235 1Department of Molecular Genetics, The Ohio State University Columbus, OH 43210 2Department of Chemistry, Indiana University Bloomington, IN 47405 3Department of Immunology and Medical Microbiology, University of Florida College of Medicine, Gainesville, FL 32610, USA
*To whom correspondence should be addressed
Received February 23, 1994. Revised April 25, 1994. Accepted April 25, 1994.
Six mutations blocking the function of a seven intron form of the mitochondrial gene encoding subunit I of cytochrome c oxidase (COXI) and mapping upstream of exon 3 were isolated and characterized. A cis-dominant mutant of the group IIA intron 1 defines a helical portion of the C1 substructure of domain 1 as essential for splicing. A trans-recessive mutant confirms that the intron 1 reading frame encodes a maturase function. A cis-dominant mutant in exon 2 was found to have no effect on the splicing of intron 1 or 2. A trans-recessive mutant, located in the group IA intron 2, demonstrates for the first time that intron 2 encodes a maturase. A genetic dissection of the five missense mutations present in the intron 2 reading frame of that strain demonstrates that the maturase defect results from one or both of the missense mutations in a newly-recognized conserved sequence called domain X.
+Present addresses: Department of Biological Sciences, Indiana University, South Bend, IN 46634
American Cyanamide Corp., Pearl River, NY 10965, USA
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