Skip Navigation

This Article
Right arrow Print PDF (8616K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (18)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Vorbrueggen, G.
Right arrow Articles by Moelling, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vorbrueggen, G.
Right arrow Articles by Moelling, K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 13 2466-2475
© 1994

MOLECULAR BIOLOGY

The carboxyterminus of human c-myb protein stimulates activated transcription in trans

Gerd Vorbrueggen, Frank Kalkbrenner+, Stephan Guehmann and Karin Moelling*

Max-Planck-Institut für Molekulare Genetik Abteilung Schuster, Ihnestrasse 73, D-14195 Berlin, Germany

* To whom correspondence should be addressed at: Institut für Medizinische Virologie.Universität Zürich, CH-8028 Zürich, Switzerland

Received April 25, 1994. Revised June 8, 1994. Accepted June 8, 1994.

The cellular c-myb gene encodes a transcription factor composed of a DNA-binding domain, a transactivating domain and a regulatory domain located at its carboxy (C-) terminus. The latter one is deleted in the transforming viral protein v-Myb. Here we show that deletion of the C-terminus of c-Myb increases the transcriptional transactivation activity of c-Myb defining it as cis-acting negative regulatory domain. Cotransfection of the Cterminus in an in vivo competition assay causes stimulation of the transcriptional activity of various vand c-Myb expression constructs in trans. The effect is dose-dependent and independent of the kind of DNAbinding domain, since c-Myb as well as GAL4-c-Myb chimaeras can be stimulated in trans. Other transcription factors, such as GAL4-VP16, GAL4, c-Jun or C/EBPß are also stimulated by the cotransfected Cterminus. In contrast, human B-Myb is not stimulated by the c-Myb C-terminus in trans. The data suggest that the C-terminus of c-Myb may interact with a cellular inhibitor which is part of the protein complex mediating activated transcription and may stimulate in trans by sequestering away such an inhibitor. Binding of c-Myb to a putative inhibitor would explain differences between c-Myb in comparison to B- and v-Myb in transcriptional regulation.

+Present address: Institut für Pharmakologie der Freien Universität Berlin, Thielallee 69/73, D-14195 Berlin, Germany


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
J. A. Iñiguez-Lluhí and D. Pearce
A Common Motif within the Negative Regulatory Regions of Multiple Factors Inhibits Their Transcriptional Synergy
Mol. Cell. Biol., August 15, 2000; 20(16): 6040 - 6050.
[Abstract] [Full Text]

Home page
 Genes Dev.Home page
A B Dash, F C Orrico, and S A Ness
The EVES motif mediates both intermolecular and intramolecular regulation of c-Myb.
Genes & Dev., August 1, 1996; 10(15): 1858 - 1869.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.