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Nucleic Acids Research, 1994, Vol. 22, No. 13 2547-2551
© 1994

RNA

The gene encoding topoisomerase II from Plasmodium falciparum

Sandra Cheesman, Sybil McAleese, Michael Goman, Delia Johnson, Paul Horrocks, Robert G. Ridley+ and Brian J. Kilbey*

Institute of Cell and Molecular Biology, University of Edinburgh King's Buildings, Mayfield Road, Edinburgh EH9 3JR, UK

*To whom correspondence should be addressed

Received April 1, 1994. Revised June 1, 1994. Accepted June 1, 1994.

The gene for topoisomerase II has been isolated from genomic libraries of strain K1 of the human malarial parasite, Plasmodium falciparum. The sequence reveals an open reading frame of 4194 nucleotides which predicts a polypeptide of 1398 amino acids. There are apparently no introns. The sequence is present as a single copy which has an identity of 47.4% and a similarity of 65.4% with its human homologue. Sequences conserved in topoisomerase II from other species are present in Pftopoisomerase II but in addition it has two adjacent asparagine-rich insertions which are unique to it. We have also detected asparagine-rich regions in the gene for PfDNA polymerase {alpha}. The gene for Pftopoisomerase II has been localised to chromosome 14 and northern analysis reveals a transcript of 5.8kb. Two independent antisera raised in mice against glutathione-Stransferase fusion proteins containing the amino terminal portion of the malarial protein detect a weak band on western blots at about 160kDa, the expected size of the protein. Use of the same antisera for immunofluorescence analysis suggests that the protein is present at all stages of intraerythrocytic growth of the parasite.

+Present address: Pharma Division, Preclinical Research, F.Hoffman-La Roche, CH5002 Basel, Switzerland


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