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Nucleic Acids Research, 1994, Vol. 22, No. 13 2612-2618
© 1994

RNA

Targeted RNA fingerprinting: the cloning of differentiallyexpressed cDNA fragments enriched for members of the zinc finger gene family

Brad Stone and Walker Wharton*

Life Sciences Division, Los Alamos National Laboratories, Los Alamos and The Department of Anatomy/Pathology, The University of New Mexico School of Medicine Albuquerque, NM, USA

*To whom correspondence should be addressed at: LS-1 , MS M888, Los Alamos National Laboratory, Los Alamos. NM 87545. USA

Received March 14, 1994. Revised May 17, 1994. Accepted May 17, 1994.

We have developed and applied a modification of an 'RNA Fingerprinting' protocol previously published by Welsh and McClelland (Nucleic Acids Research 19: 5275 – 5279 1991) such that cDNA fragments which are both differentially-expressed and enriched for members of a specific gene family can readily be identified. cDNA fragments were amplified with an arbitrary primer initially used in the reverse transcription reaction in combination with a member of a primer set which corresponded to a conserved region within a specific gene family. This technique was used to isolate cDNAs encoding a recently described protein kinase as well as an unknown gene that contained a zinc finger. Several other known genes that contained a zinc finger domain and that were differentially-expressed were also isolated.


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