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Nucleic Acids Research, 1994, Vol. 22, No. 14 2744-2751
© 1994

CHEMISTRY

A plant scaffold attached region detected close to a T-DNA integration site is active in mammalian cells

Antje Dietz*, Volker Kay1, Thomas Schlake1, Jörg Landsmann and Jürgen Bode1

Institut für Biochemie und Pflanzenvirologie, Biologische Bundesanstalt Messeweg 11/12, D-38104 Braunschweig 1Gesellschaft für Biotechnologische Forschung mbH, Genetik von Eukaryonten Mascheroder Weg 1, D-38124 Braunschweig-Stöckheim, Germany

*To whom correspondence should be addressed

Received May 2, 1994. Revised June 16, 1994. Accepted June 16, 1994.

Integration of foreign genes into plant genomes by the Agrobacterium T-DNA transfer system has been considered to occur at random. It has been speculated that the chromosomal structure of the integration site might affect the expression pattern of the introduced genes. To gain insight into the molecular structure of T-DNA integration sites and its possible impact on gene expression, we have examined plant DNA sequences in the vicinity of T-DNA borders. Analysis of a transgenic petunia plant containing a chloramphenicol acetyltransferase (CAT) gene regulated by the hemoglobin promoter (PAR) from Parasponia andersonii revealed a scaffold attachment region (SAR) close to one T-DNA end. In addition to having strong binding affinities for both animal and plant nuclear scaffolds this petunia SAR element is as active in mammalian cells as the authentic elements from mammalian sources.


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