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Nucleic Acids Research, 1994, Vol. 22, No. 15 2915-2921
© 1994

CHEMISTRY

Preparation of a DNA matrix via an electrqchemically directed copolymerization of pyrrole and oligonucleotides bearing a pyrrole group

Thierry Livache, André Roget, Emmanuel Dejean1, Christelle Barthet1, Gérard Bidan1 and Robert Teoule1,*

CIS Bio international, Laboratoire des Sondes Moleculaires BP 32, 91192 Gif-sur-Yvette 1CEA/Département de Recherche Fondamentale sur la Matiére Condensee/SESAM/EM 17 rue des Martyrs, 38054 Grenoble Cedex 9, France

*To whom correspondence should be addressed

Received June 1, 1994. Accepted June 30, 1994.

A new methodology for the preparation of adressed DNA matrices is described. The process includes an electrochemically directed copolymerization of pyrrole and oligonucleotides bearing on their 5' end a pyrrole moiety introduced by phosphoramidite chemistry. The electro-controlledsynthesis of the copolymer (polypyrrole) gives, in one step, a solid conducting film deposited on the surface of an electrode. The resulting polymer consists of pyrrole chains bearing covalently linked oligonucleotide. The polymer growth is limited to the electrode surface, so that it is possible to prepare a DNA matrix on a multiple electrode device by successive copolymerizations. A support bearing four oligonucleotides was used to detect three ras mutations on asynthetic DNA fragment


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