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Nucleic Acids Research, 1994, Vol. 22, No. 15 2976-2981
© 1994

MOLECULAR BIOLOGY

Tandemly repeated transgenes of the human minisatellite MS32 (D1S8), with novel mouse gamma satellite integration

Maxine J. Allen*, Alec J. Jeffreys, M.Azim Surani1, Sheila Barton1, Michael LNorris2 and Andrew Collick

Department of Genetics, University of Leicester Leicester LE1 7RH 1Wellcome CRC Institute of Developmental Biology Cambridge CB2 1QR 2AFRC Institute Babraham, Cambridge CB2 4AT, UK

*To whom correspondence should be addressed

Received May 16, 1994. Revised June 22, 1994. Accepted June 22, 1994.

The human hypervariable minisatellite MS32 has a well characterised internal repeat unit array and high mutation rates have been observed at this locus. Analysis of MS32 mutants has shown that male germline mutations are polarised to one end of the array and frequently involve complex gene conversionlike events, suggesting that tandem repeat instability may be modulated by cis-acting sequences flanking the locus. In order to investigate the processes affecting MS32 mutation rate and mechanism, we have created transgenic mice harbouring an MS32 allele. Here we describe the organisation of eight transgenic insertions. Analysis of these transgenic loci by MVRPCR and structural analysis of the junctions between mouse flanking DNA and the transgenic loci has shed light on mechanisms of integration and rearrangement of the tandem repeated transgenes. Sequence analysis of the mouse DNA flanking these transgenes has shown that 5 of the 8 insertions have integrated into mouse gamma satellite repeated sequence. This suggets a non-random integration of the MS32 transgene construct into the mouse genome.


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