Nucleic Acids Research, 1994, Vol. 22, No. 15 3138-3146
© 1994
MOLECULAR BIOLOGY |
Mcp and Fab-7: molecular analysis of putative boundaries of cis-regulatory domains in the bithorax complex of Drosophila melanogaster
Department of Zoology and Animal Biology, University of Geneva 154 rte de Malagnou, 1224 Geneva, Switzerland 1Institute of Genetics, Biological Research Center, Hungarian Academy of Sciences PO Box 521, 6701 Szeged 2Hungary and department of Biology, Princeton University Princeton, NJ 08544, USA
*To whom correspondence should be addressed
Received April 8, 1994. Revised June 21, 1994. Accepted June 21, 1994.
A very large cis-regulatory region of –300 kb is responsible for the complex patterns of expression of the three homeotic genes of the bithorax complex Ubx, abd-A and Abd-B. This region can be subdivided in nine parasegment-specific regulatory subunits. Recent genetic and molecular analysis has revealed the existence of two novel cis-regulatory elements Mcp and Fab-7. Mcp is located between iab-4 and iab-5, the parasegment-specific regulatory subunits which direct Abd-B in parasegments 9 and 10. Similarly, Fab-7 is located between iab-6 and iab-7, the parasegment 11 and 12-specific regulatory units. Mcp and Fab-7 appear to function as domain boundaries that separate adjacent cis-regulatory units. We report the analysis of two new Mcp mutant deletions (Mcp27 and Mcp8116) that allow us to localize sequences essential for boundary function to a – 0 .4 kb DNA segment. These essential sequences closely coincide to a –0.3 kb nuclease hypersensitive region in chromatin. We also show that sequences contributing to the Fab-7 boundary appear to be spread over a larger stretch of DNA, but like Mcp have an unusual chromatin structure.
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