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Nucleic Acids Research, 1994, Vol. 22, No. 16 3317-3321
© 1994

GENOME STRUCTURE AND MAPPING

Characterization of minisatellites in Arabidopsis thaliana with sequence similarity to the human minisatellite core sequence

S. Tourmente*, J.M. Deragon, J. Lafleuriel, S. Tutois, T. Pélissier, C. Cuvillier, M.C. Espagnol and G. Picard

GDR 977 Biomove CNRS, Université Blaise Pascal 24 Avenue des Landais, 63177 Aubiere Cedex, France

*To whom correspondence should be addressed

Received June 16, 1994. Revised June 25, 1994. Accepted June 25, 1994.

A strategy based on random PCR amplification was used to isolate new repetitive elements of Arabidopsis thaliana. One of the random PCR product analyzed by this approach contained a tandem repetitive minisatellite sequence composed of 33 bp repeated units. The genomic locus corresponding to this PCR product was isolated by screening a lambda genomic library. New related loci were also isolated from the genomic library by screening with a 14 mer oligonucleotide representing a region conserved among the different repeated units. Alignment of the consensus sequence for each minisatellite locus allowed the definition of an Arabidopsis thaliana core sequence that shows strong sequence similarities with the human core sequence and with the generalized recombination signal Chi of Escherichia coli. The minisatellites were tested for their ability to detect polymorphism, and their chromosomal position was established.


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