Skip Navigation

This Article
Right arrow Print PDF (3301K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (105)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ushida, C.
Right arrow Articles by Muto, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ushida, C.
Right arrow Articles by Muto, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 16 3392-3396
© 1994

RNA

tRNA-like structures in 10Sa RNAs of Mycoplasma capricolum and Bacillus subtilis

Chisato Ushida, Hyouta Himeno, Tsuneaki Watanabe1 and Akira Muto2

Department of Biology, Faculty of Science, Hirosaki University Bunkyo-cho, Hirosaki 036 1Center for Gene Research, Nagoya University Furo-cho, Chikusa-ku, Nagoya 464-01, Japan

*To whom correspondence should be addressed

Received May 5, 1994. Revised July 13, 1994. Accepted July 13, 1994.

The stable RNAs, whose sequences are homologous to 10Sa RNA of Escherichia coli, have been isolated from Mycoplasma capricolum and Bacillus subtilis, both belonging to the Gram-positive bacterial group. The total nucleotide sequences of the RNAs have been determined by partial RNA sequencing and DNA sequencing of their genes. A comparison of the sequences, together with those of other bacterial 10Sa RNAs so far known, has shown that the 5'- and 3'-end sequences are well conserved among species, while the central parts reveal little homologies. Unexpectedly, the conserved 5'- and 3'-regions can be folded in a common tRNA-like structure containing an amino acidacceptor stem and a T{Psi}C-stem/loop. The 3'-terminal CCA sequence of B.subtilis 10Sa RNA is not encoded on the DNA, but is added after transcription. Furthermore, the RNA is aminoacylatable with alanine in vitro, and binds to the 70S ribosome in vitro.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
D. Kurita, R. Sasaki, A. Muto, and H. Himeno
Interaction of SmpB with ribosome from directed hydroxyl radical probing
Nucleic Acids Res., December 18, 2007; 35(21): 7248 - 7255.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
T. Konno, D. Kurita, K. Takada, A. Muto, and H. Himeno
A functional interaction of SmpB with tmRNA for determination of the resuming point of trans-translation
RNA, October 1, 2007; 13(10): 1723 - 1731.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
N. Ivanova, M. Lindell, M. Pavlov, L. Holmberg Schiavone, E. G. H. Wagner, and M. Ehrenberg
Structure probing of tmRNA in distinct stages of trans-translation
RNA, May 1, 2007; 13(5): 713 - 722.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
K. Takada, C. Takemoto, M. Kawazoe, T. Konno, K. Hanawa-Suetsugu, S. Lee, M. Shirouzu, S. Yokoyama, A. Muto, and H. Himeno
In vitro trans-translation of Thermus thermophilus: Ribosomal protein S1 is not required for the early stage of trans-translation
RNA, April 1, 2007; 13(4): 503 - 510.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Saguy, R. Gillet, P. Skorski, S. Hermann-Le Denmat, and B. Felden
Ribosomal protein S1 influences trans-translation in vitro and in vivo
Nucleic Acids Res., April 1, 2007; 35(7): 2368 - 2376.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
C. I. Montero, D. L. Lewis, M. R. Johnson, S. B. Conners, E. A. Nance, J. D. Nichols, and R. M. Kelly
Colocation of Genes Encoding a tRNA-mRNA Hybrid and a Putative Signaling Peptide on Complementary Strands in the Genome of the Hyperthermophilic Bacterium Thermotoga maritima.
J. Bacteriol., October 1, 2006; 188(19): 6802 - 6807.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Asano, D. Kurita, K. Takada, T. Konno, A. Muto, and H. Himeno
Competition between trans-translation and termination or elongation of translation
Nucleic Acids Res., October 4, 2005; 33(17): 5544 - 5552.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
N. Ivanova, M. Y. Pavlov, E. Bouakaz, M. Ehrenberg, and L. H. Schiavone
Mapping the interaction of SmpB with ribosomes by footprinting of ribosomal RNA
Nucleic Acids Res., June 21, 2005; 33(11): 3529 - 3539.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
O. V. Shpanchenko, M. I. Zvereva, P. V. Ivanov, E. Y. Bugaeva, A. S. Rozov, A. A. Bogdanov, M. Kalkum, L. A. Isaksson, K. H. Nierhaus, and O. A. Dontsova
Stepping Transfer Messenger RNA through the Ribosome
J. Biol. Chem., May 6, 2005; 280(18): 18368 - 18374.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
I. K. Wower, C. Zwieb, and J. Wower
Contributions of Pseudoknots and Protein SmpB to the Structure and Function of tmRNA in trans-Translation
J. Biol. Chem., December 24, 2004; 279(52): 54202 - 54209.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. M. Sharkady and K. P. Williams
A third lineage with two-piece tmRNA
Nucleic Acids Res., August 23, 2004; 32(15): 4531 - 4538.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Konno, T. Takahashi, D. Kurita, A. Muto, and H. Himeno
A minimum structure of aminoglycosides that causes an initiation shift of trans-translation
Nucleic Acids Res., August 4, 2004; 32(14): 4119 - 4126.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Hallier, N. Ivanova, A. Rametti, M. Pavlov, M. Ehrenberg, and B. Felden
Pre-binding of Small Protein B to a Stalled Ribosome Triggers trans-Translation
J. Biol. Chem., June 18, 2004; 279(25): 25978 - 25985.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
Y. JACOB, E. SEIF, P.-O. PAQUET, and B. F. LANG
Loss of the mRNA-like region in mitochondrial tmRNAs of jakobids
RNA, April 1, 2004; 10(4): 605 - 614.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. A. B. Kolkman and E. Ferrari
The fate of extracellular proteins tagged by the SsrA system of Bacillus subtilis
Microbiology, February 1, 2004; 150(2): 427 - 436.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
T. Takahashi, T. Konno, A. Muto, and H. Himeno
Various Effects of Paromomycin on tmRNA-directed trans-Translation
J. Biol. Chem., July 18, 2003; 278(30): 27672 - 27680.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Corvaisier, V. Bordeau, and B. Felden
Inhibition of Transfer Messenger RNA Aminoacylation and trans-Translation by Aminoglycoside Antibiotics
J. Biol. Chem., April 18, 2003; 278(17): 14788 - 14797.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
Y. YAMAMOTO, T. SUNOHARA, K. JOJIMA, T. INADA, and H. AIBA
SsrA-mediated trans-translation plays a role in mRNA quality control by facilitating degradation of truncated mRNAs
RNA, April 1, 2003; 9(4): 408 - 418.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
K. C. Keiler and L. Shapiro
tmRNA Is Required for Correct Timing of DNA Replication in Caulobacter crescentus
J. Bacteriol., January 15, 2003; 185(2): 573 - 580.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. Laslett, B. Canback, and S. Andersson
BRUCE: a program for the detection of transfer-messenger RNA genes in nucleotide sequences
Nucleic Acids Res., August 1, 2002; 30(15): 3449 - 3453.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Hanawa-Suetsugu, M. Takagi, H. Inokuchi, H. Himeno, and A. Muto
SmpB functions in various steps of trans-translation
Nucleic Acids Res., April 1, 2002; 30(7): 1620 - 1629.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
K. Hanawa-Suetsugu, V. Bordeau, H. Himeno, A. Muto, and B. Felden
Importance of the conserved nucleotides around the tRNA-like structure of Escherichia coli transfer-messenger RNA for protein tagging
Nucleic Acids Res., November 15, 2001; 29(22): 4663 - 4673.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
J. Wower, I. K. Wower, B. Kraal, and C. W. Zwieb
Quality Control of the Elongation Step of Protein Synthesis by tmRNP
J. Nutr., November 1, 2001; 131(11): 2978S - 2982.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
T. Wiegert and W. Schumann
SsrA-Mediated Tagging in Bacillus subtilis
J. Bacteriol., July 1, 2001; 183(13): 3885 - 3889.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
B. Felden, C. Massire, E. Westhof, J. F. Atkins, and R. F. Gesteland
Phylogenetic analysis of tmRNA genes within a bacterial subgroup reveals a specific structural signature
Nucleic Acids Res., April 1, 2001; 29(7): 1602 - 1607.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. C. Keiler, L. Shapiro, and K. P. Williams
tmRNAs that encode proteolysis-inducing tags are found in all known bacterial genomes: A two-piece tmRNA functions in Caulobacter
PNAS, July 5, 2000; 97(14): 7778 - 7783.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Lin-Chao, C.-L. Wei, and Y.-T. Lin
RNase E is required for the maturation of ssrA RNA and normal ssrA RNA peptide-tagging activity
PNAS, October 26, 1999; 96(22): 12406 - 12411.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
J. Withey and D. Friedman
Analysis of the Role of trans-Translation in the Requirement of tmRNA for lambda immP22 Growth in Escherichia coli
J. Bacteriol., April 1, 1999; 181(7): 2148 - 2157.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. Schimmel and R. Alexander
Diverse RNA substrates for aminoacylation: Clues to origins?
PNAS, September 1, 1998; 95(18): 10351 - 10353.
[Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
J. B. Andersen, C. Sternberg, L. K. Poulsen, S. P. Bjørn, M. Givskov, and S. Molin
New Unstable Variants of Green Fluorescent Protein for Studies of Transient Gene Expression in Bacteria
Appl. Envir. Microbiol., June 1, 1998; 64(6): 2240 - 2246.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. D. Roche and R. T. Sauer
Identification of Endogenous SsrA-tagged Proteins Reveals Tagging at Positions Corresponding to Stop Codons
J. Biol. Chem., July 20, 2001; 276(30): 28509 - 28515.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.