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Nucleic Acids Research, 1994, Vol. 22, No. 17 3640-3659
© 1994

Articles

Effect of site-specific modification on restriction endonucleases and DNA modification methyltransferases

Michael McClelland*, Michael Nelson1 and Eberhard Raschke2

California Institute of Biological Research 11099 North Torrey Pines Road, La Jolla, CA 92037 1 Megabase Research Products 4129 Holdrege, Lincoln, NE 68503, USA 2 Universitat Bonn Kirschallee 1, D-53115 Bonn, Germany

* To whom correspondence should be addressed

Restriction endonucleases have site-specific interactions with DNA that can often be inhibited by site-specific DNA methylation and other site-specific DNA modifications. However, such inhibition cannot generally be predicted. The empirically acquired data on these effects are tabulated for over 320 restriction endonucleases. In addition, a table of known sitespecific DNA modification methyltransferases and their specificities is presented along with EMBL database accession numbers for cloned genes.


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