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Nucleic Acids Research, 1994, Vol. 22, No. 18 3671-3680
© 1994

STRUCTURAL BIOLOGY

Structural characterization of intrinsically curved AT-rich DNA sequences

Pilar Carrera and Fernando Azorin*

Departament de Biologia Molecular i Cellular, Centre d'Investigació i Desenvolupament-CSIC Jordi Girona Salgado 18-26, 08034 Barcelona, Spain

*To whom correspondence should be addressed

Received June 30, 1994. Revised August 10, 1994. Accepted August 10, 1994.

AT-rich DNA sequences other than AnTm tracts (n $ m≥4) are known to be intrinsically curved. The AATAT-element constitutes one known example of these sequences. In this paper, the elucidation of the structural basis of the curvature induced by this sequence element was addressed. As judged by the patterns of cleavage by the hydroxyl radical and DNase I, the AATAT sequence shows a narrow minor groove. Furthermore, the 5' adenine residue of the AA dinucleotide contained within the sequence is hyperreactive to diethylpyrocarbonate. Similar structural properties are shown by several sequences inducing intrinsic DNA curvature, such as an A5-tract or the closely related ATAAT, AATATA and TAATAT sequences, which are also shown here to induce curvature. On the other hand, other related sequences, such as TATAA and ATATA, that do not induce curvature, show different structural characteristics.


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