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Nucleic Acids Research, 1994, Vol. 22, No. 18 3737-3741
© 1994

MOLECULAR BIOLOGY

Inefficient excision of uracil from loop regions of DNA oligomers by E.coli uracil DNA glycosylase

N.Vinay Kumar and U. Varshney*

Centre for Genetic Engineering, Indian Institute of Science Bangalore 560012, India

*To whom correspondence should be addressed.

Revised July 26, 1994. Accepted July 26, 1994.

Kinetic parameters for uracil DNA glycosylase (E.coli)- catalysed excision of uracil from DNA oligomers containing dUMP in different structural contexts were determined. Our results show that single-stranded oligonucleotides (unstructured) are used as somewhat better substrates than the double-stranded oligonucleotides. This is mainly because of the favourable Vmax value of the enzyme for singlestranded substrates. More interestingly, however, we found that uracil release from loop regions of DNA hairpins is extremely inefficient. The poor efficiency with which uracil is excised from loop regions is a result of both increased Km and lowered Vmax values. This observation may have significant implications in uracil DNA glycosylase-directed repair of DNA segments that can be extruded as hairpins. In addition, these studies are useful in designing oligonucleotides for various applications in DNA research where the use of uracil DNA glycosylase is sought.


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