Skip Navigation

This Article
Right arrow Print PDF (5231K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (109)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cobb, B. D.
Right arrow Articles by CIarkson, J. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cobb, B. D.
Right arrow Articles by CIarkson, J. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 18 3801-3805
© 1994

METHODS

A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods

Benjamin D. Cobb* and John M. CIarkson

Microbial Pathogenicity Group, School of Biology and Biochemistry, University of Bath Bath BA2 7AY, UK

*To whom correspondence should be addressed

Received March 30, 1994. Revised July 20, 1994. Accepted July 20, 1994.

Taguchi methods are used widely as the basis for development trials during industrial process design. Here, we describe their suitability for optimisation of the PCR. Unlike conventional strategies, these arrays revealed the effects and interactions of specific reaction components simultaneously using just a few reactions, negating the need for extensive experimental investigation. Reaction components which effected product yield were easily determined. In addition, this technique was applied to the qualitative investigation of RAPD-PCR profiles, where optimisation of the size and distribution of a number of products was determined.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
R. Andreson, T. Mols, and M. Remm
Predicting failure rate of PCR in large genomes
Nucleic Acids Res., June 1, 2008; 36(11): e66 - e66.
[Abstract] [Full Text] [PDF]

Home page
 BioinformaticsHome page
F. Miura, C. Uematsu, Y. Sakaki, and T. Ito
A novel strategy to design highly specific PCR primers based on the stability and uniqueness of 3'-end subsequences
Bioinformatics, December 15, 2005; 21(24): 4363 - 4370.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
S. Haynes, A. C. Darby, T. J. Daniell, G. Webster, F. J. F. van Veen, H.C.J. Godfray, J. I. Prosser, and A. E. Douglas
Diversity of Bacteria Associated with Natural Aphid Populations
Appl. Envir. Microbiol., December 1, 2003; 69(12): 7216 - 7223.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
C. M. Waterfall and B. D. Cobb
Single tube genotyping of sickle cell anaemia using PCR-based SNP analysis
Nucleic Acids Res., December 1, 2001; 29(23): e119 - e119.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
B. Hammer, A. Moter, O. Kahl, G. Alberti, and U. B. Gobel
Visualization of Borrelia burgdorferi sensu lato by fluorescence in situ hybridization (FISH) on whole-body sections of Ixodes ricinus ticks and gerbil skin biopsies
Microbiology, June 1, 2001; 147(6): 1425 - 1436.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Beaulieu, G. P. Larson, L. Geller, S. D. Flanagan, and T. G. Krontiris
PCR candidate region mismatch scanning: adaptation to quantitative, high-throughput genotyping
Nucleic Acids Res., March 1, 2001; 29(5): 1114 - 1124.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Wu, B. R. Emery, and D. T. Carrell
In Vitro Growth, Maturation, Fertilization, and Embryonic Development of Oocytes from Porcine Preantral Follicles
Biol Reprod, January 1, 2001; 64(1): 375 - 381.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
B. B. Ward, D. P. Martino, M. C. Diaz, and S. B. Joye
Analysis of Ammonia-Oxidizing Bacteria from Hypersaline Mono Lake, California, on the Basis of 16S rRNA Sequences
Appl. Envir. Microbiol., July 1, 2000; 66(7): 2873 - 2881.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
P. Gomez-De-Leon, J. I. Santos, J. Caballero, D. Gomez, L. E. Espinosa, I. Moreno, D. Piñero, and A. Cravioto
Genomic Variability of Haemophilus influenzae Isolated from Mexican Children Determined by Using Enterobacterial Repetitive Intergenic Consensus Sequences and PCR
J. Clin. Microbiol., July 1, 2000; 38(7): 2504 - 2511.
[Abstract] [Full Text]

Home page
 Appl. Environ. Microbiol.Home page
S. Poussier, P. Vandewalle, and J. Luisetti
Genetic Diversity of African and Worldwide Strains of Ralstonia solanacearum as Determined by PCR-Restriction Fragment Length Polymorphism Analysis of the hrp Gene Region
Appl. Envir. Microbiol., May 1, 1999; 65(5): 2184 - 2194.
[Abstract] [Full Text]

Home page
 Infect. Immun.Home page
J. R. Naglik, G. Newport, T. C. White, L. L. Fernandes-Naglik, J. S. Greenspan, D. Greenspan, S. P. Sweet, S. J. Challacombe, and N. Agabian
In Vivo Analysis of Secreted Aspartyl Proteinase Expression in Human Oral Candidiasis
Infect. Immun., May 1, 1999; 67(5): 2482 - 2490.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
F. Lo Presti, S. Riffard, F. Vandenesch, and J. Etienne
Identification of Legionella Species by Random Amplified Polymorphic DNA Profiles
J. Clin. Microbiol., November 1, 1998; 36(11): 3193 - 3197.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
S. Appuhamy, J. G. Coote, J. C. Low, and R. Parton
PCR Methods for Rapid Identification and Characterization of Actinobacillus seminis Strains
J. Clin. Microbiol., March 1, 1998; 36(3): 814 - 817.
[Abstract] [Full Text]

Home page
 J. Exp. Med.Home page
Q. Chen, A. Barragan, V. Fernandez, A. Sundstrom, M. Schlichtherle, A. Sahlen, J. Carlson, S. Datta, and M. Wahlgren
Identification of Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) as the Rosetting Ligand of the Malaria Parasite P. falciparum
J. Exp. Med., January 5, 1998; 187(1): 15 - 23.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
K H Roux
Optimization and troubleshooting in PCR.
Genome Res., April 1, 1995; 4(5): S185 - S194.
[PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.