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Nucleic Acids Research, 1994, Vol. 22, No. 19 3928-3929
© 1994

MOLECULAR BIOLOGY

New restriction endonuclease CviRI cleaves DNA at TG/CA sequences

Aiwen Jin, Yanping Zhang1, Yuannan Xia2, Elbert Traylor1, Michael Nelson and James L. Van Ettenl*

Megabase Research Products 4129 Holdrege, Number Two, Lincoln, NE 68503 1Department of Plant Pathology, University of Nebraska Lincoln, NE 68583-0722 2BioNebraska, Inc. 3820 NW 46th Street, Lincoln, NE 68524, USA

*To whom correspondence should be addressed

Received June 6, 1994. Revised August 8, 1994. Accepted August 8, 1994.

A new type II restriction endonuclease, CviRI, was isolated from virus XZ-6E infected chlorella cells. CviRI is the first restriction endonuclease to recognize the sequence 5'-TGCA-3' and cleaves DNA between the G and C residues to produce blunt-end termini. Methylation of the adenine or cytosine in 5'-TGCA-3' sequences prevents CviRI cleavage. Due to its sequence specificity, CviRI may be especially useful for detecting mutant alleles of many heritable human genetic diseases.


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