Skip Navigation

This Article
Right arrow Print PDF (776K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (40)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Case-Green, S. C.
Right arrow Articles by Southern, E. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Case-Green, S. C.
Right arrow Articles by Southern, E. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 2 131-136
© 1994

CHEMISTRY

Studies on the base pairing properties of deoxyinosine by solid phase hybridisation to oligonucleotides

Stephen C. Case-Green and Edwin M. Southern

Department of Biochemistry, University of Oxford South Parks Road, Oxford 0X1 3QU, UK

Received November 17, 1993. Accepted December 9, 1993.

Extensive analyses of the base-pairing properties of deoxyinosine to A, C, G and T were carried out by measuring the hybridisation of oligonucleotides with deoxyinosine In various positions to complementary sets of oligonucleotides made as an array on the surface of a glass microscope slide. With deoxyinosine In Internal positions, results are consistent with previous studies, showing a preferential order for pairing of I-C > I-A > I-G - I-T. With two adjacent deoxylnosines In the centre of the ollgonucleotide, the order In duplex yield Is CC > CA > AA > AC > GC > GA > CG > TA > TC > CT = AG > AT > GT > TT. With deoxyinosine at the ends of the oligonucleotide, we find that at the 3' end there is the same order in duplex yield as for the deoxyinosine In Internal positions, though with lower discrimination between the bases. When hybridisation is carried out in TMACI there Is little base pairing discrimination with deoxyinosine, or indeed any of the four natural bases at the 5' end. Changing the cation to Na+ Increased discrimination slightly.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
N. E. Watkins Jr and J. SantaLucia Jr
Nearest-neighbor thermodynamics of deoxyinosine pairs in DNA duplexes
Nucleic Acids Res., November 1, 2005; 33(19): 6258 - 6267.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
H. B. GAMPER JR., K. ARAR, A. GEWIRTZ, and Y.-M. HOU
Unrestricted accessibility of short oligonucleotides to RNA
RNA, September 1, 2005; 11(9): 1441 - 1447.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M. D. Wyatt and L. D. Samson
Influence of DNA structure on hypoxanthine and 1,N6-ethenoadenine removal by murine 3-methyladenine DNA glycosylase
Carcinogenesis, May 1, 2000; 21(5): 901 - 908.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. Saparbaev, J.-C. Mani, and J. Laval
Interactions of the human, rat, Saccharomyces cerevisiae and Escherichia coli 3-methyladenine-DNA glycosylases with DNA containing dIMP residues
Nucleic Acids Res., March 15, 2000; 28(6): 1332 - 1339.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.