Skip Navigation

This Article
Right arrow Print PDF (889K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (15)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Papp, P. P.
Right arrow Articles by Chattoraj, D. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Papp, P. P.
Right arrow Articles by Chattoraj, D. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 2 152-157
© 1994

MOLECULAR BIOLOGY

Missing-base and ethylation interference footprinting of P1 plasmid replication initiator

Peter P. Papp+ and Dhruba K. Chattoraj*

Laboratory of Biochemistry, National Cancer Institute NIH, Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received November 4, 1993. Accepted December 2, 1993.

RepA, the replication Initiator protein of plasmid P1, binds to specific 19 bp sequences on the plasmid DNA. Earlier footprinting studies with dimethylsulfate Identified the guanlnes that contact RepA through the major groove of DNA. In this study, base elimination was used to Identify the contribution of all four bases to the binding reaction. Depurinatlon and depyrimldatlon of any base In the neighborhood of the contacting guanlnes was found to decrease RepA binding. These results are consistent with the notion that RepA contacts bases of two consecutive major grooves on the same face of DNA. We also observed that depurination but not methylation of three guanines (G3, G8 and G9) affected binding. We identified the DNA phosphate groups (3 in the top strand, one of which mapped between G8 and G9, and 4 in the bottom strand, one of which was adjacent to C3) that strongly Interfered with RepA binding upon ethylation. These results Indicate that certain bases (e.g. G3, G8 and G9) may not contact RepA directly but contribute to base and backbone contacts by maintaining proper structure of the binding site.

plus;Present address: Department of Biology, Carlton University, Ottawa, Ontario K1S 5B6, Canada.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
G. Krings and D. Bastia
Molecular Architecture of a Eukaryotic DNA Replication Terminus-Terminator Protein Complex
Mol. Cell. Biol., November 1, 2006; 26(21): 8061 - 8074.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Sharma, B. K. Sathyanarayana, J. G. Bird, J. R. Hoskins, B. Lee, and S. Wickner
Plasmid P1 RepA Is Homologous to the F Plasmid RepE Class of Initiators
J. Biol. Chem., February 13, 2004; 279(7): 6027 - 6034.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. D. Schneider
Strong minor groove base conservation in sequence logos implies DNA distortion or base flipping during replication and transcription initiation
Nucleic Acids Res., December 1, 2001; 29(23): 4881 - 4891.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
I. G. Lyakhov, P. N. Hengen, D. Rubens, and T. D. Schneider
The P1 phage replication protein RepA contacts an otherwise inaccessible thymine N3 proton by DNA distortion or base flipping
Nucleic Acids Res., December 1, 2001; 29(23): 4892 - 4900.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
P. Pál Papp, P. Élö, S. Semsey, and L. Orosz
Footprinting Studies of Specific Complexes Formed by RepA, a Replication Initiator of Plasmid pCU1, and Its Binding Site
J. Bacteriol., October 1, 2000; 182(19): 5409 - 5415.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.