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Nucleic Acids Research, 1994, Vol. 22, No. 2 194-199
© 1994

GENOME STRUCTURE AND MAPPING

A rapid and simple method to isolate and characterize highly polymorphic markers from the centromeric regions of the human chromosomes

A.M. Laurent, B. Marcais, M. Muleris1 and G. Roizès*

UPR 9008 CNRS and U 249 INSERM Montpellier 1URA 620 CNRS Paris, France

*To whom correspondence should be addressed

Received October 18, 1993. Accepted December 15, 1993.

Using oligonucleotide primers complementary to the 3' ends of either the Alu or the L1Hs consensus sequences In conjunction with a primer complementary to alpha satellite subsets specific to different human chromosomes, It was possible to detect and characterize polymorphisms originating from the microsatellltes which are often present downstream these repetitive elements. The methodology does not require cloning, sequencing or synthesis of specific primers. Centromeric location was confirmed by linkage analysis, In situ hybridization and sequencing. The method Is proposed for the generation of polymorphic markers from all centromeric regions.


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