Nucleic Acids Research, 1994, Vol. 22, No. 20 4183-4186
© 1994
MOLECULAR BIOLOGY |
Identity of the RNA-binding protein K of hnRNP particles with protein H16, a sequence-specific single strand DNAbinding protein
Institut Jacques Monod 2 place Jussieu, 75251 Paris 05, France
*To whom correspondence should be addressed
Received June 24, 1994. Revised August 31, 1994. Accepted August 31, 1994.
Protein H16, which we have identified previously in mammalian cell lines, binds in vitro to two single stranded DNA sites on the late strand of the early promoter of SV40. It has no other single strand binding site in the SV40 genome and does not bind to double stranded DNA. In vitro, H16 can be shown to stimulate strongly the activity of purified RNA polymerase II. Here we have purified this 70 kDa protein from cultured monkey cells and have sequenced three of its tryptic peptides. The analysis indicates that H16 is the simian homolog of human protein K, a nuclear RNA-binding protein found in heterogeneous nuclear ribonucleoprotein (hnRNP) particles, which contains a KH domain present in several proteins including the fragile X mental retardation gene product (FMR1). The binding affinities of protein K/H16 for RNA and DNA were subsequently compared in detail. They showed that under conditions where K/H16 binds strongly to its single stranded DNA site, it binds very weakly to the corresponding RNA sequence. This result suggests a possible shuttling of the protein from RNA to DNA during processes which involve opening of the DNA double helix.
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