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Nucleic Acids Research, 1994, Vol. 22, No. 20 4335-4341
© 1994

MOLECULAR BIOLOGY

Kin17, a mouse nuclear zinc finger protein that binds preferentially to curved DNA

Alexander Mazin$, Tatiana Timchenko1, Josiane Ménissier-de Murcia1,ø, Valérie Schreiber1,ø, Jaime F. Angulo2,§, Murcia Gilbert de1,ø and Raymond Devoret*

G.E. Mutagenése et Cancérogenese, Institut Curie, Bâtiment 110, Centre Universitaire, F-91405 Orsay 1UPR 9003, Cancérogenèse et Mutagenèse Moléculaire et Structurale, Institut de Biologie Moléculaire et Cellulaire CNRS, 15 rue Descartes, F-67084 Strasbourg 2G.E.Mutagenèse et Cancerogénèse Laboratoire d'Enzymologie CNRS, F-91198 Gif sur Yvette, France

*To whom correspondence should be addressed

Received April 5, 1994. Revised August 24, 1994. Accepted August 24, 1994.

Kin17 is a 45 kDa protein encoded by the KIN17 gene located on mouse chromosome 2, band A. The kin17 amino acid sequence predicts two domains, which were shown to be functional: (i) a bipartite nuclear localization signal (NLS) that can drive the protein to the cell nucleus, (ii) a bona fide zinc finger of the C2H2 type. The zinc finger is involved in kin17 binding to double-stranded DNA since a mutant deleted of the zinc finger, kin17{delta}1, showed reduced binding. Singlestranded DNA was bound poorly by kin17. Interestingly, we found that kin17 protein showed preferential binding to curved DNA from either pBR322 or synthetic oligonucleotides. Binding of kin17 to a non-curved DNA segment increased after we had inserted into it a short curved synthetic oligonucleotide. Kin17{delta}2, a mutant deleted of 110 amino acids at the C-terminal end, still exhibited preferential binding to curved DNA and so did kin17{delta}1, suggesting that a domain recognizing curved DNA is located in the protein core.

$Section of Microbiology, Hutchison Hall, University of California at Davis, DAVIS, CA 95616-8665, USA,

§Laboratoire de Cytométrie Départ. Pathol. & Toxicol. Experimentales, D.S.V., C. E. A., BP 6, F-92265 Fontenay Aux Roses and

øUPR 9003, Cancérogenese et Mutagenése Moléculaire et Structurale, Pôle API ESBS/CNRS, Boulevard Sébastien Brandt, F-67400 Illkirch, France


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