Nucleic Acids Research, 1994, Vol. 22, No. 21 4419-4431
© 1994
MOLECULAR BIOLOGY |
Interactions among regualators of RNA abundance characterized using RNA fingerprinting by arbitrarily primed PCR
California Institute of Biological Research 11099 North Torrey Pines Road, La Jolla, CA 92037, USA
*To whom correspondence should be addressed
Received July 18, 1994. Revised September 1, 1994. Accepted September 1, 1994.
Using RNA fingerprinting by arbitrarily primed PCR it is possible to infer convergent transcript regulatory pathways from the coordinate behavior of subsets of anonymous transcripts without cloning any genes. The number of transcripts in each response category can be estimated. The same may be true for differential display. We demonstrate these claims by treating a cell line with two known modulators of RNA abundance, transforming growth factor-ß (TGFß) and cycloheximide (CX), used together and alone. The responses of over 1700 anonymous transcripts were monitored under these three conditions and in an untreated control. Eight of the twenty-seven [33] possible transcript response categories were observed among 86 differentially expressed transcripts. For example, CX stabilizes or induces as many as 2.7% of transcripts of which about one third do not accumulate when TGFß is also present. This intersection may reflect CX stabilization or induction of an important class of RNAs that otherwise usually have short half-lives. We predict that RNAs in this class constitute the majority of transcripts targeted for rapid down regulation in response to TGFß and perhaps most other natural transcriptional modulators.
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