Nucleic Acids Research, 1994, Vol. 22, No. 21 4462-4469
© 1994
MOLECULAR BIOLOGY |
Structure, expression and chromosomal localization of human p80-coilin gene
Department of molecular and Experimental Medicine, The Scripps Research Institute 10666 N. Torrey Pines Road, La Jolla, CA 92037 1Department of Genetics and Center for Human Genetics, Case Western Reserve University 10900 Euclid Avenue, Cleveland, OH 44106-4955, USA
*To whom correspondence should be addressed
Received June 22, 1994. Revised August 10, 1994. Accepted August 10, 1994.
Coiled bodies (CBs) are non-capsular nuclear bodies with a diameter of 0.3 - 1µm and appear to be composed of coiled fibrils. Human autoantibodies to CBs recognize an 80-kD nuclear protein highly enriched in CBs, and this protein has been named p80-coilin. CBs are known to assemble and disassemble during the cell cycle, with the highest number of CBs occurring at mid to late G1 where p80-coilin is assembled into several small nuclear body-like structures. In S and G2 phases, CBs become larger and their number decreases and often they are undetectable during mitosis. Using a human autoantibody as a probe for expression cloning, we initially isolated a partial cDNA encoding p80-coilin. In this report, the 5' end of the complete cDNA for p80-coilin was obtained using the 5'-RACE (rapid amplification of cDNA ends) methodology. The size of the reconstructed full-length cDNA corresponds to the 2.7-kb mRNA detected in Northern blot analysis. The complete p80-coilin protein consists of 576 amino acids with a predicted molecular mass of 62,608. A putative p80-coilin pseudogene was also detected during the rescreening of p80-coilin cDNA. To confirm the validity of the cDNA sequence, three overlapping genomic DNA clones representing the human p80-coilin gene were selected for further analysis. The complete gene for p80-coilin contains 7 exons spanning -25kb. Sequence analysis of exons 1 and 2 in genomic DNA clones confirmed the accuracy of the 5' cDNA sequence derived from the 5'-RACE procedure. Furthermore, the human p80-coilin gene was localized to chromosome 17q22–23 by fluorescence in situ hybridization.
+Present address: Division of Rheumatology, Escola Paulista de Medicina, Rua Botucatu 740, Sao Paulo, SP 04023, Brazil
EMBL U06632
[GenBank]
; GenBank Lo6522
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