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Nucleic Acids Research, 1994, Vol. 22, No. 21 4470-4476
© 1994

MOLECULAR BIOLOGY

Transfer of a constitutive viral pormoter–cystic fibrosis transmembrance conductance regulator cDNA to human epithelial cells conveys resistance to down-regulation of cAMP-regulated Cl– secretion in the presence of inflammatory stimuli

Nobuyuki Kobayashi1, Eugene R. Rosenthal1, Kunihiko Yoshimura1 and Ronald G. Crustal1,2,*

1Pulmonary Branch, National Heart, Lung, and Blood Institute, National Institutes of Health Bethesda, MD 20892 2Division of Pulmonary and Critical Care Medicine, Cornell University Medical College New York, NY, USA

*To whom correspondence should be addressed at: Pulmonary Branch, Building 10, Room 6D03, National Institutes of Heatlth, Bethesda, MD 20892. USA

Received June 22, 1994. Revised September 12, 1994. Accepted September 12, 1994.

The expression of the cystic fibrosis transmembrane conductance regulator (CFTR) gene can be downregulated by inflammatory stimuli such as phorbol myristate acetate (PMA). Since the respiratory manifestations of cystic fibrosis (CF) are characterized by intense chronic airway inflammation very early in life, successful gene therapy for CF will require that expression of the transferred normal CFTR gene be resistant to down-regulation by inflammatory mediators. To evaluate the concept that a viral promoter– human CFTR cDNA unit would be resistant to this form of down-regulation, a retrovirus promoter (5' long terminal repeat of the Moloney murine leukemia virus) – human CFTR cDNA unit was transferred to T84 human colon carcinoma cell line using a retrovirus vector. Exposure of the retrovirus-modified T84 cells to PMA resulted in down-regulation of the endogenous CFTR mRNA transcripts (6.5 kb), but did not affect the level of exogenous CFTR transcripts (8.0 kb). Importantly, in parallel with the persistence of the exogenous CFTR transcripts, the modified cells still maintained cAMP-regulated Cl- secretion in the presence of PMA. These in vitro data suggest that a constitutive viral promoter –CFTR cDNA unit should be resistant to modulation by inflammatory stimuli, a likely requirement for successful gene therapy for CF.


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