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Nucleic Acids Research, 1994, Vol. 22, No. 21 4489-4496
© 1994

MOLECULAR BIOLOGY

NF-{varkappa}B-independent activation of ß-interferon expression in mouse F9 embryonal carcinoma cells

Matthew J.C. Ellis+ and Stephen Goodbourn*

Gene Expression Laboratory, Imperial Cancer Research Fund 44 Lincoln's Inn Fields, London WC2A 3PX, UK

*To whom correspondence should be addressed at: Division of Biochemistry, Department of Cellual and Molecular Sciences, St Geroge's Hospital Medical school, University of London Cranmer, Terrace, London SW17 0RE UK

Received June 10, 1994. Revised September 23, 1994. Accepted September 23, 1994.

We have examined the behaviour of the ß-interferon promoter in mouse F9 embryonal carcinoma cells. In undifferentiated cells, the ß-interferon promoter is not responsive to dsRNA or to Sendai virus. In cells stimulated to differentiate into parietal endoderm by treatment with retinoic acid, the ß-interferon promoter responds to both inducers, but only Sendai virus can activate the transcription factor NF-{varkappa}B previously thought to be essential for ß-interferon induction. Differentiated F9 cells therefore present an unprecedented situation in which induction of the ß- interferon gene does not require NF-{varkappa}B. In addition to these differences, induction by dsRNA, but not by Sendai virus, is significantly enhanced by a pretreatment with interferon (priming). These observations suggest that paramyxo-viruses can participate in ß- interferon induction in a manner that is distinct from a simple generator of dsRNA. Analysis of the promoter requirements for induction in differentiated F9 cells suggests that induction is brought about by a novel mechanism using the currently identified regulatory domains.

+Present address: Vincent T.Lombardi Cancer Research Center, Georgetown University, 3800 Reservoir Road Northwest, Washington, DC 20007, USA


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