Nucleic Acids Research, 1994, Vol. 22, No. 22 4566-4573
© 1994
MOLECULAR BIOLOGY |
Molecular cloning of cDNA encoding human DNA helicase Q1 Which has homology to Escherichia coli Rec Q helicase and localization of the gene at chromosome 12p12
Department of Physiological Chemistru, Faculty of Pharmaceutical Sciences, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113 1Cellular Physiology Laboratory, The Institute of Physical and Chemical Research (RIKEN) Wako, Saitama 351-01 2Division of Human Genome Research and Gene bank, The Institute of Physical and Cehmical Research (RIKEN) Tsukuba Science City, Ibaraki 305 3Laboratory of Biological Chemistry, Faculty of Bioresources, Mie university 1515 Kamihama, Tsu, Mie 514, Japan
*To whom correspondence should be addressed
Received August 26, 1994. Revised October 10, 1994. Accepted October 10, 1994.
A complementary DNA encoding DNA-dependent ATPase Q1 possessing DNA helicase activity, which is the major DNA-dependent ATPase in human cell extracts, was cloned from a cDNA library of human KB cells. The predicted amino acid sequence has seven consecutive motifs conserved in the RNA and DNA helicase super family and DNA helicase Q1 belongs to DEXH helicase family. A homology search indicated that helicase Q1 had 47% homology in the seven conserved regions with Escherichia coli RecQ protein. Three RNA bands of 4.0, 3.3, and 2.2 kilobases were detected in HeLa cells by Northern blotting. Analysis of the genomic DNA indicated the presence of a homologous gene in mouse cells. The DNA helicase Q1 gene was localized on the short arm of human chromosome 12 at 12p12.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. V. Bugreev, R. M. Brosh Jr., and A. V. Mazin RECQ1 Possesses DNA Branch Migration Activity J. Biol. Chem., July 18, 2008; 283(29): 20231 - 20242. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. M. Brosh Jr and V. A. Bohr Human premature aging, DNA repair and RecQ helicases Nucleic Acids Res., December 3, 2007; 35(22): 7527 - 7544. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sharma, D. J. Stumpo, A. S. Balajee, C. B. Bock, P. M. Lansdorp, R. M. Brosh Jr., and P. J. Blackshear RECQL, a Member of the RecQ Family of DNA Helicases, Suppresses Chromosomal Instability Mol. Cell. Biol., March 1, 2007; 27(5): 1784 - 1794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Z. Bachrati, R. H. Borts, and I. D. Hickson Mobile D-loops are a preferred substrate for the Bloom's syndrome helicase. Nucleic Acids Res., January 1, 2006; 34(8): 2269 - 2279. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Kato and H. Inoue Growth Defect and Mutator Phenotypes of RecQ-Deficient Neurospora crassa Mutants Separately Result From Homologous Recombination and Nonhomologous End Joining During Repair of DNA Double-Strand Breaks Genetics, January 1, 2006; 172(1): 113 - 125. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. W. Lee, R. Kusumoto, K. M. Doherty, G.-X. Lin, W. Zeng, W.-H. Cheng, C. von Kobbe, R. M. Brosh Jr., J.-S. Hu, and V. A. Bohr Modulation of Werner Syndrome Protein Function by a Single Mutation in the Conserved RecQ Domain J. Biol. Chem., November 25, 2005; 280(47): 39627 - 39636. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. LeRoy, R. Carroll, S. Kyin, M. Seki, and M. D. Cole Identification of RecQL1 as a Holliday junction processing enzyme in human cell lines Nucleic Acids Res., October 31, 2005; 33(19): 6251 - 6257. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Petkovic, T. Dietschy, R. Freire, R. Jiao, and I. Stagljar The human Rothmund-Thomson syndrome gene product, RECQL4, localizes to distinct nuclear foci that coincide with proteins involved in the maintenance of genome stability J. Cell Sci., September 15, 2005; 118(18): 4261 - 4269. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sharma, J. A. Sommers, S. Choudhary, J. K. Faulkner, S. Cui, L. Andreoli, L. Muzzolini, A. Vindigni, and R. M. Brosh Jr. Biochemical Analysis of the DNA Unwinding and Strand Annealing Activities Catalyzed by Human RECQ1 J. Biol. Chem., July 29, 2005; 280(30): 28072 - 28084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Doherty, S. Sharma, L. A. Uzdilla, T. M. Wilson, S. Cui, A. Vindigni, and R. M. Brosh Jr. RECQ1 Helicase Interacts with Human Mismatch Repair Factors That Regulate Genetic Recombination J. Biol. Chem., July 29, 2005; 280(30): 28085 - 28094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Hu, X. Lu, E. Barnes, M. Yan, H. Lou, and G. Luo Recql5 and Blm RecQ DNA Helicases Have Nonredundant Roles in Suppressing Crossovers Mol. Cell. Biol., May 1, 2005; 25(9): 3431 - 3442. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cui, D. Arosio, K. M. Doherty, R. M. Brosh Jr, A. Falaschi, and A. Vindigni Analysis of the unwinding activity of the dimeric RECQ1 helicase in the presence of human replication protein A Nucleic Acids Res., April 19, 2004; 32(7): 2158 - 2170. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mashimo, M. Kawata, and K. Yamamoto Roles of the RecJ and RecQ proteins in spontaneous formation of deletion mutations in the Escherichia coli K12 endogenous tonB gene Mutagenesis, July 1, 2003; 18(4): 355 - 363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Wang, M. Seki, Y. Narita, T. Nakagawa, A. Yoshimura, M. Otsuki, Y.-i. Kawabe, S. Tada, H. Yagi, Y. Ishii, et al. Functional Relation among RecQ Family Helicases RecQL1, RecQL5, and BLM in Cell Growth and Sister Chromatid Exchange Formation Mol. Cell. Biol., May 15, 2003; 23(10): 3527 - 3535. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cui, R. Klima, A. Ochem, D. Arosio, A. Falaschi, and A. Vindigni Characterization of the DNA-unwinding Activity of Human RECQ1, a Helicase Specifically Stimulated by Human Replication Protein A J. Biol. Chem., January 10, 2003; 278(3): 1424 - 1432. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kawasaki, S. Maruyama, M. Nakayama, K. Matsumoto, and T. Shibata Drosophila melanogaster RECQ5/QE DNA helicase: stimulation by GTP binding Nucleic Acids Res., September 1, 2002; 30(17): 3682 - 3691. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mohaghegh, J. K. Karow, R. M. Brosh Jr, V. A. Bohr, and I. D. Hickson The Bloom's and Werner's syndrome proteins are DNA structure-specific helicases Nucleic Acids Res., July 1, 2001; 29(13): 2843 - 2849. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Hu, S. Beresten, A. van Brabant, T.-Z. Ye, P.-P. Pandolfi, F. B. Johnson, L. Guarente, and N. A. Ellis Evidence for BLM and Topoisomerase III{{alpha}} interaction in genomic stability Hum. Mol. Genet., June 1, 2001; 10(12): 1287 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 O. Bischof, S.-H. Kim, J. Irving, S. Beresten, N. A. Ellis, and J. Campisi Regulation and Localization of the Bloom Syndrome Protein in Response to DNA Damage J. Cell Biol., April 16, 2001; 153(2): 367 - 380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Mohaghegh and I. D. Hickson DNA helicase deficiencies associated with cancer predisposition and premature ageing disorders Hum. Mol. Genet., April 1, 2001; 10(7): 741 - 746. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Ohsugi, Y. Tokutake, N. Suzuki, T. Ide, M. Sugimoto, and Y. Furuichi Telomere repeat DNA forms a large non-covalent complex with unique cohesive properties which is dissociated by Werner syndrome DNA helicase in the presence of replication protein A Nucleic Acids Res., September 15, 2000; 28(18): 3642 - 3648. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Miyajima, M. Seki, F. Onoda, M. Shiratori, N. Odagiri, K. Ohta, Y. Kikuchi, Y. Ohno, and T. Enomoto Sgs1 Helicase Activity Is Required for Mitotic but Apparently Not for Meiotic Functions Mol. Cell. Biol., September 1, 2000; 20(17): 6399 - 6409. [Abstract] [Full Text]
|
|
|
|
|
|
J.-C. Shen and L. A. Loeb Werner syndrome exonuclease catalyzes structure-dependent degradation of DNA Nucleic Acids Res., September 1, 2000; 28(17): 3260 - 3268. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Huang, S. Beresten, B. Li, J. Oshima, N. A. Ellis, and J. Campisi Characterization of the human and mouse WRN 3'->5' exonuclease Nucleic Acids Res., June 15, 2000; 28(12): 2396 - 2405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Brosh Jr, J. K. Karow, E. J. White, N. D. Shaw, I. D. Hickson, and V. A. Bohr Potent inhibition of Werner and Bloom helicases by DNA minor groove binding drugs Nucleic Acids Res., June 15, 2000; 28(12): 2420 - 2430. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Kim, J. Lee, and H.-S. Koo Functional characterization of Caenorhabditis elegans DNA topoisomerase III{alpha} Nucleic Acids Res., May 1, 2000; 28(9): 2012 - 2017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Shimamoto, K. Nishikawa, S. Kitao, and Y. Furuichi Human RecQ5{beta}, a large isomer of RecQ5 DNA helicase, localizes in the nucleoplasm and interacts with topoisomerases 3{alpha} and 3{beta} Nucleic Acids Res., April 1, 2000; 28(7): 1647 - 1655. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Walpita, A. W. Plug, N. F. Neff, J. German, and T. Ashley Bloom's syndrome protein, BLM, colocalizes with replication protein A in meiotic prophase nuclei of mammalian spermatocytes PNAS, May 11, 1999; 96(10): 5622 - 5627. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Kusano, M. E. Berres, and W. R. Engels Evolution of the RECQ Family of Helicases: A Drosophila Homolog, Dmblm, Is Similar to the Human Bloom Syndrome Gene Genetics, March 1, 1999; 151(3): 1027 - 1039. [Abstract] [Full Text]
|
|
|
|
|
|
R. J. Bennett, J. A. Sharp, and J. C. Wang Purification and Characterization of the Sgs1 DNA Helicase Activity of Saccharomyces cerevisiae J. Biol. Chem., April 17, 1998; 273(16): 9644 - 9650. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. G. Harmon and S. C. Kowalczykowski RecQ helicase, in concert with RecA and SSB proteins, initiates and disrupts DNA recombination Genes & Dev., April 15, 1998; 12(8): 1134 - 1144. [Abstract] [Full Text]
|
|
|
|
|
|
J. K. Karow, R. K. Chakraverty, and I. D. Hickson The Bloom's Syndrome Gene Product Is a 3'-5' DNA Helicase J. Biol. Chem., December 5, 1997; 272(49): 30611 - 30614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Miyamoto, N. Imamoto, T. Sekimoto, T. Tachibana, T. Seki, S. Tada, T. Enomoto, and Y. Yoneda Differential Modes of Nuclear Localization Signal (NLS) Recognition by Three Distinct Classes of NLS Receptors J. Biol. Chem., October 17, 1997; 272(42): 26375 - 26381. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. A. Sinclair, K. Mills, and L. Guarente Accelerated Aging and Nucleolar Fragmentation in Yeast sgs1 Mutants Science, August 29, 1997; 277(5330): 1313 - 1316. [Abstract] [Full Text]
|
|
|
|
|
|
K. Hanada, T. Ukita, Y. Kohno, K. Saito, J.-i. Kato, and H. Ikeda RecQ DNA helicase is a suppressor of illegitimate recombination in Escherichia coli PNAS, April 15, 1997; 94(8): 3860 - 3865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-i. Kawabe, M. Seki, T. Seki, W.-S. Wang, O. Imamura, Y. Furuichi, H. Saitoh, and T. Enomoto Covalent Modification of the Werner's Syndrome Gene Product with the Ubiquitin-related Protein, SUMO-1 J. Biol. Chem., July 7, 2000; 275(28): 20963 - 20966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-i. Kawabe, D. Branzei, T. Hayashi, H. Suzuki, T. Masuko, F. Onoda, S.-J. Heo, H. Ikeda, A. Shimamoto, Y. Furuichi, et al. A Novel Protein Interacts with the Werner's Syndrome Gene Product Physically and Functionally J. Biol. Chem., June 1, 2001; 276(23): 20364 - 20369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. M. Brosh Jr., J.-L. Li, M. K. Kenny, J. K. Karow, M. P. Cooper, R. P. Kureekattil, I. D. Hickson, and V. A. Bohr Replication Protein A Physically Interacts with the Bloom's Syndrome Protein and Stimulates Its Helicase Activity J. Biol. Chem., July 28, 2000; 275(31): 23500 - 23508. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Bischof, S. Galande, F. Farzaneh, T. Kohwi-Shigematsu, and J. Campisi Selective Cleavage of BLM, the Bloom Syndrome Protein, during Apoptotic Cell Death J. Biol. Chem., April 6, 2001; 276(15): 12068 - 12075. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Karow, A. Constantinou, J.-L. Li, S. C. West, and I. D. Hickson The Bloom's syndrome gene product promotes branch migration of Holliday junctions PNAS, June 6, 2000; 97(12): 6504 - 6508. [Abstract] [Full Text] [PDF]
|
|