Nucleic Acids Research, 1994, Vol. 22, No. 23 4932-4936
© 1994
MOLECULAR BIOLOGY |
Role of a small RNA pol II subunit in TATA to transcription start site spacing
Institute for Cell Biology, Swiss Federal Insitute of Techonology ETH-Hönggerberg, CH-8093 Sürich, Switzeraland 1Department of Genetics, SK–50, University of Washington Seattle, WA 98195, USA
*To whom correpsondence should be addressed at: Department of Biochemistry and Molecular Biology, Lederfe Graduate Research Center, University of Massachusetrs, Amberst, MA 01003, USA
Received September 6, 1994. Revised October 17, 1994. Accepted October 17, 1994.
The yeast shi mutation affects the spacing between the TATA promoter element and transcription initiation sites; for the H2B and ADH1 genes, a series of start sites located –50 –80 bp downstream of TATA is used in addition to the wild-type initiation sites located at around 100 bp from TATA (1). Here, the yeast SHI wildtype gene has been isolated by complementation and shown to be identical to RPB9, the gene encoding a small subunit of RNA polymerase II. A point mutation in the shi gene, changing a cysteine residue in a putative zinc ribbon motif into a phenylalanine residue, was demonstrated to permit the observed usage of upstream initiation sites. Deletion of the non-essential SHI gene also results in usage of upstream initiation sites and causes conditional growth defects.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. H. Jenks, T. W. O'Rourke, and D. Reines Properties of an Intergenic Terminator and Start Site Switch That Regulate IMD2 Transcription in Yeast Mol. Cell. Biol., June 15, 2008; 28(12): 3883 - 3893. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Chen, C. Ruggiero, and S. Li Yeast Rpb9 Plays an Important Role in Ubiquitylation and Degradation of Rpb1 in Response to UV-Induced DNA Damage Mol. Cell. Biol., July 1, 2007; 27(13): 4617 - 4625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Li, B. Ding, R. Chen, C. Ruggiero, and X. Chen Evidence that the Transcription Elongation Function of Rpb9 Is Involved in Transcription-Coupled DNA Repair in Saccharomyces cerevisiae Mol. Cell. Biol., December 15, 2006; 26(24): 9430 - 9441. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. N. Kuehner and D. A. Brow Quantitative Analysis of in Vivo Initiator Selection by Yeast RNA Polymerase II Supports a Scanning Model J. Biol. Chem., May 19, 2006; 281(20): 14119 - 14128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Seshadri, A. G. McArthur, M. L. Sogin, and R. D. Adam Giardia lamblia RNA Polymerase II: AMANITIN-RESISTANT TRANSCRIPTION J. Biol. Chem., July 18, 2003; 278(30): 27804 - 27810. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D.-Y. Zhang, D. J. Carson, and J. Ma The role of TFIIB-RNA polymerase II interaction in start site selection in yeast cells Nucleic Acids Res., July 15, 2002; 30(14): 3078 - 3085. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. L. Mueller and J. A. Jaehning Ctr9, Rtf1, and Leo1 Are Components of the Paf1/RNA Polymerase II Complex Mol. Cell. Biol., April 1, 2002; 22(7): 1971 - 1980. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Cramer, D. A. Bushnell, J. Fu, A. L. Gnatt, B. Maier-Davis, N. E. Thompson, R. R. Burgess, A. M. Edwards, P. R. David, and R. D. Kornberg Architecture of RNA Polymerase II and Implications for the Transcription Mechanism Science, April 28, 2000; 288(5466): 640 - 649. [Abstract] [Full Text]
|
|
|
|
|
|
W. Hausner, U. Lange, and M. Musfeldt Transcription Factor S, a Cleavage Induction Factor of the Archaeal RNA Polymerase J. Biol. Chem., April 21, 2000; 275(17): 12393 - 12399. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Hemming and A. M. Edwards Yeast RNA Polymerase II Subunit RPB9. MAPPING OF DOMAINS REQUIRED FOR TRANSCRIPTION ELONGATION J. Biol. Chem., January 28, 2000; 275(4): 2288 - 2294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-J. Cho and S. Buratowski Evidence That Transcription Factor IIB Is Required for a Post-assembly Step in Transcription Initiation J. Biol. Chem., September 3, 1999; 274(36): 25807 - 25813. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. A. Hawkes and S. G. E. Roberts The Role of Human TFIIB in Transcription Start Site Selection in Vitro and in Vivo J. Biol. Chem., May 14, 1999; 274(20): 14337 - 14343. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Chédin, M. Riva, P. Schultz, A. Sentenac, and C. Carles The RNA cleavage activity of RNA polymerase III is mediated by an essential TFIIS-like subunit and is important for transcription termination Genes & Dev., December 15, 1998; 12(24): 3857 - 3871. [Abstract] [Full Text]
|
|
|
|
|
|
T. S. Pardee, C. S. Bangur, and A. S. Ponticelli The N-terminal Region of Yeast TFIIB Contains Two Adjacent Functional Domains Involved in Stable RNA Polymerase II Binding and Transcription Start Site Selection J. Biol. Chem., July 10, 1998; 273(28): 17859 - 17864. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hampsey Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 465 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Acker, M. de Graaff, I. Cheynel, V. Khazak, C. Kedinger, and M. Vigneron Interactions between the Human RNA Polymerase II Subunits J. Biol. Chem., July 4, 1997; 272(27): 16815 - 16821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. E. Awrey, R. G. Weilbaecher, S. A. Hemming, S. M. Orlicky, C. M. Kane, and A. M. Edwards Transcription Elongation through DNA Arrest Sites. A MULTISTEP PROCESS INVOLVING BOTH RNA POLYMERASE II SUBUNIT RPB9 AND TFIIS J. Biol. Chem., June 6, 1997; 272(23): 14747 - 14754. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z Wang and R G Roeder Three human RNA polymerase III-specific subunits form a subcomplex with a selective function in specific transcription initiation. Genes & Dev., May 15, 1997; 11(10): 1315 - 1326. [Abstract] [PDF]
|
|
|
|
|
|
G Orphanides, T Lagrange, and D Reinberg The general transcription factors of RNA polymerase II. Genes & Dev., November 1, 1996; 10(21): 2657 - 2683. [PDF]
|
|
|
|
|
|
S. A. Hemming, D. B. Jansma, P. F. Macgregor, A. Goryachev, J. D. Friesen, and A. M. Edwards RNA Polymerase II Subunit Rpb9 Regulates Transcription Elongation in Vivo J. Biol. Chem., November 3, 2000; 275(45): 35506 - 35511. [Abstract] [Full Text] [PDF]
|
|