Nucleic Acids Research, 1994, Vol. 22, No. 23 5079-5084
© 1994
GENOME STRUCTURE AND MAPPING |
Analyiss of the micronuclear B type surface protein gene in Paramecium tetruaurelia
Department of Biochemistry 1153 Biochemistry Building, Purdue University West Lafayette, In 47907, USA
*To whom correspondence shoul be addressed
Received June 24, 1994. Revised October 10, 1994. Accepted October 10, 1994.
The micronuclear DNA of Paramecium contains sequence that are precisely excised during the formation of the macronuclear (somatic) genome. In this paper we show that four eliminated sequences ranging in size from 28 to 416 base pairs, are present in or near the micronuclear copy of the B surface protein gene. Each excised sequence is bounded by the dinucleotide 5'TdA-3'. Comparison of the micronuclear B gene with the previously determined micronuclear sequence of the A surface protein gene shows that although the positions of at least three of the eliminated sequences are conserved in both genes, the sequences are highly divergent. Transformation of vegetative macronuclei with fragments of the micronuclear B gene results in replication and maintenance of the DNA but the micronuclear specific sequences are not removed. PRevious studies have shown that the correct incorporation of the B gene into the new marconucleus requires copies of the macronuclear B gene in the old macronucleus. Using macronuclear transformation, we show that the micronuclear B gene can substitute for the macronuclear B gene with regard to its role in DNA processing. This suggests that the macronuclear DNA is not acting as a guide for the excision of the micronuclear specific sequences
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