Nucleic Acids Research, 1994, Vol. 22, No. 24 5184-5189
© 1994
Articles |
Two separate sites contribute to AP-1 activation of the promoter for 3-hydroxy-3-methylglutaryl coenzyme A synthase
Department of Molecular Biology and Biochemistry, University of California at Irvine, Irvine CA 92717-3900, USA
*To whom correspondence should be addressed
Received October 12, 1994. Accepted October 24, 1994.
Metabolic flux into the mevalonate pathway is regulated by end product repression and cell growth. In the experiments reported here the transcrlptlonal promoter for an early enzyme of the pathway, 3-hydroxy-3-methylglutaryl coenzyme A synthase, Is shown to be activated by the growth stimulatory agent tetraphorbol acetate (TPA). We show that TPA has a direct stimulatory action on the promoter and further that this Is mediated by the AP-1 transcription factor. In addition, we show that there are two separate cls-actlng sites that bind AP-1 and both are required for maximal stimulation. We further show that in AP-1-deficient cells ectoplc expression of AP-1 stimulates synthetic promoters containing two copies of each synthase element upstream of a minimal promoter. The physiological rationale of having both end product repression and direct activation by growth stimulatory cues is discussed.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  D. Ma, C. L. Nutt, P. Shanehsaz, X. Peng, D. N. Louis, and D. M. Kaetzel Autocrine Platelet-Derived Growth Factor-Dependent Gene Expression in Glioblastoma Cells Is Mediated Largely by Activation of the Transcription Factor Sterol Regulatory Element Binding Protein and Is Associated with Altered Genotype and Patient Survival in Human Brain Tumors Cancer Res., July 1, 2005; 65(13): 5523 - 5534. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Datta and T. F. Osborne Activation Domains from Both Monomers Contribute to Transcriptional Stimulation by Sterol Regulatory Element-binding Protein Dimers J. Biol. Chem., February 4, 2005; 280(5): 3338 - 3345. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Dooley, M. K. Bennett, and T. F. Osborne A Critical Role for cAMP Response Element-binding Protein (CREB) as a Co-activator in Sterol-regulated Transcription of 3-Hydroxy-3-methylglutaryl Coenzyme A Synthase Promoter J. Biol. Chem., February 26, 1999; 274(9): 5285 - 5291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. A. Dooley, S. Millinder, and T. F. Osborne Sterol Regulation of 3-Hydroxy-3-Methylglutaryl-coenzyme A Synthase Gene through a Direct Interaction Between Sterol Regulatory Element Binding Protein and the Trimeric CCAAT-binding Factor/Nuclear Factor Y J. Biol. Chem., January 16, 1998; 273(3): 1349 - 1356. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. D. Strahl, H.-J. Huang, N. R. Pedersen, J. C. Wu, B. R. Ghosh, and W. L. Miller Two Proximal Activating Protein-1-Binding Sites Are Sufficient to Stimulate Transcription of the Ovine Follicle-Stimulating Hormone-{beta} Gene Endocrinology, June 1, 1997; 138(6): 2621 - 2631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Jackson, J. Ericsson, T. F. Osborne, and P. A. Edwards NF-Y Has a Novel Role in Sterol-dependent Transcription of Two Cholesterogenic Genes J. Biol. Chem., September 15, 1995; 270(37): 21445 - 21448. [Abstract] [Full Text] [PDF]
|
|