Nucleic Acids Research, 1994, Vol. 22, No. 24 5223-5228
© 1994
Articles |
Splicing factor SF3a60 is the mammalian homologue of PRP9 of S.cerevisiae: the conserved zinc finger-like motif is functionally exchangeable in vivo
Deépartement de Biologie Cellulaire, Universiteé de Geneéve, 30 quai Ernest-Ansermet, CH-1211 Geneéve 4, Switzerland 1Deépartement de Biologie Moleéculaire, Institut Pasteur 25 rue du Dr Roux, F-75724 Paris Cedex 15, France 2Pharma Research, Department of Molecular and Cell Biology Ciba-Geigy Ltd, CH-4002 Basel, Switzerland
*To whom correspondence should be addressed
Received September 23, 1994. Accepted October 28, 1994.
A cDNA encoding the 60 kDa subunit of mammalian splicing factor SF3a has been isolated. The deduced protein sequence reveals a 30% Identity to the PRP9 splicing protein of the yeast S.cerevisiae. The highest homology is present In a zinc finger-like region In the C-terminal domain of both proteins. The PRP9 zinc finger-like motif has been replaced by the equivalent region of mammalian SF3a60. The chlmerlc protein rescues the temperature-sensitive phenotype of the prp9-1 mutant strain demonstrating that not only the structure but also the function of this domain has been conserved during evolution.
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