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Nucleic Acids Research, 1994, Vol. 22, No. 24 5229-5234
© 1994

Articles

Modified RNA sequence pools for in vitro selection

Yun Lin, Qiu Qiu, Stanley C. Gill and Sumedha D. Jayasena*

NeXagen, Inc. 2860 Wilderness Place, Suite 200, Boulder, CO 80301, USA

*To whom correspondence should be addressed

Received September 19, 1994. Accepted October 31, 1994.

We report the use of modified RNA, In which the 2'-OH group of pyrimldlnes is replaced by a 2'-amino (2'-NH2) group to Identify high affinity ligands specific for human neutrophll elastase (HNE) by In vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and LI + ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.


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