Skip Navigation

This Article
Right arrow Print PDF (2102K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (78)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lin, Y.
Right arrow Articles by Jayasena, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lin, Y.
Right arrow Articles by Jayasena, S. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 24 5229-5234
© 1994

Articles

Modified RNA sequence pools for in vitro selection

Yun Lin, Qiu Qiu, Stanley C. Gill and Sumedha D. Jayasena*

NeXagen, Inc. 2860 Wilderness Place, Suite 200, Boulder, CO 80301, USA

*To whom correspondence should be addressed

Received September 19, 1994. Accepted October 31, 1994.

We report the use of modified RNA, In which the 2'-OH group of pyrimldlnes is replaced by a 2'-amino (2'-NH2) group to Identify high affinity ligands specific for human neutrophll elastase (HNE) by In vitro selection. Compared to unmodified RNA the 2'-NH2-modified RNA ligands show enhanced stability in human serum and urine. Use of RNase T1 cleavage data in the presence of K+ and LI + ions suggests that the modified RNA ligands selected for HNE form an intermolecular G-quartet structure.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
F. Jarosch, K. Buchner, and S. Klussmann
In vitro selection using a dual RNA library that allows primerless selection
Nucleic Acids Res., July 19, 2006; 34(12): e86 - e86.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
D. FAULHAMMER, B. ESCHGFALLER, S. STARK, P. BURGSTALLER, W. ENGLBERGER, J. ERFURTH, F. KLEINJUNG, J. RUPP, S. D. VULCU, W. SCHRODER, et al.
Biostable aptamers with antagonistic properties to the neuropeptide nociceptin/orphanin FQ
RNA, March 1, 2004; 10(3): 516 - 527.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Vater, F. Jarosch, K. Buchner, and S. Klussmann
Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach: Tailored-SELEX
Nucleic Acids Res., November 1, 2003; 31(21): e130 - e130.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. E. Lee, A. Sidorov, T. Gourlain, N. Mignet, S. J. Thorpe, J. A. Brazier, M. J. Dickman, D. P. Hornby, J. A. Grasby, and D. M. Williams
Enhancing the catalytic repertoire of nucleic acids: a systematic study of linker length and rigidity
Nucleic Acids Res., April 1, 2001; 29(7): 1565 - 1573.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Respir. Cell Mol. Bio.Home page
Q.-L. Ying and S. R. Simon
DNA from Bronchial Secretions Modulates Elastase Inhibition by alpha 1-Proteinase Inhibitor and Oxidized Secretory Leukoprotease Inhibitor
Am. J. Respir. Cell Mol. Biol., October 1, 2000; 23(4): 506 - 513.
[Abstract] [Full Text]

Home page
 Journal of Bioactive and Compatible PolymersHome page
N. Teramoto, Y. Imanishi, and Y. Ito
In Vitro Selection of Ligase Ribozymes Containing 2'-Amino Groups
Journal of Bioactive and Compatible Polymers, July 1, 2000; 15(4): 297 - 308.
[Abstract] [PDF]

Home page
 Clin. Chem.Home page
S. D. Jayasena
Aptamers: An Emerging Class of Molecules That Rival Antibodies in Diagnostics
Clin. Chem., September 1, 1999; 45(9): 1628 - 1650.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Kraus, W. James, and A. N. Barclay
Cutting Edge: Novel RNA Ligands Able to Bind CD4 Antigen and Inhibit CD4+ T Lymphocyte Function
J. Immunol., June 1, 1998; 160(11): 5209 - 5212.
[Abstract] [Full Text] [PDF]

Home page
 Journal of Bioactive and Compatible PolymersHome page
Y. Ito, N. Teramoto, N. Kawazoe, K. Inada, and Y. Imanishi
Modified Nucleic Acid for Systematic Evolution of RNA Ligands by Exponential Enrichment
Journal of Bioactive and Compatible Polymers, April 1, 1998; 13(2): 114 - 123.
[Abstract]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. P. Williams, X.-H. Liu, T. N. M. Schumacher, H. Y. Lin, D. A. Ausiello, P. S. Kim, and D. P. Bartel
Bioactive and nuclease-resistant L-DNA ligand of vasopressin
PNAS, October 14, 1997; 94(21): 11285 - 11290.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Gold, D. Brown, Y.-y. He, T. Shtatland, B. S. Singer, and Y. Wu
From oligonucleotide shapes to genomic SELEX: Novel biological regulatory loops
PNAS, January 7, 1997; 94(1): 59 - 64.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. S. Bishop, J. K. Guy-Caffey, J. O. Ojwang, S. R. Smith, M. E. Hogan, P. A. Cossum, R. F. Rando, and N. Chaudhary
Intramolecular G-quartet Motifs Confer Nuclease Resistance to a Potent Anti-HIV Oligonucleotide
J. Biol. Chem., March 8, 1996; 271(10): 5698 - 5703.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Gold and L. Gold
Oligonucleotides as Research, Diagnostic, and Therapeutic Agents
J. Biol. Chem., June 9, 1995; 270(23): 13581 - 13584.
[Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.