Nucleic Acids Research, 1994, Vol. 22, No. 24 5310-5320
© 1994
Articles |
RAP1 stimulates single- to double-strand association of yeast telomeric DNA: implications for telomere - telomere interactions
ISREC (Swiss Institute for Experimental Cancer Research) 155 Chemin des Boveresses, CH-1066 Epalinges/Lausanne, Switzerland
*To whom correspondence should be addressed
+Present addresses: Ecote Normale Supérieure de Lyon. 46 Allee d'Italie, F-69364 Lyon, France
lnstitut fur Zdilbiologie. ETH-HOnggerbcrg. CH-8093 Zurich, Switzerland
Received August 31, 1994. Accepted November 2, 1994.
Repressor Activator Protein 1 (RAP1) of Saccharo-myces cerevlslae Is an abundant nuclear protein Implicated In telomere length maintenance, transactiv-atlon, and In the establishment of silent chromatln domains. The RAP1 binding site 5' of the yeast HIS4 gene Is also a region of hyperrecombinatlon In melosls. We report here that as RAP1 binds Its recognition consensus, it appears to untwist double-stranded DNA, which we detect as the Introduction of a negative supercoil in clrcularizatlon assays. Coincident with the RAP1 -dependent untwisting, we observe stimulation of the association of a single-stranded yeast telomeric sequence with its homologous double-stranded sequence In a supercolled plasmld. This unusual distortion of the DNA double helix by RAP1 may contribute to the RAP1-dependent enhancement of recombination rates and promote non-duplex strand Interactions at telomeres.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Negrini, V. Ribaud, A. Bianchi, and D. Shore DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation Genes & Dev., February 1, 2007; 21(3): 292 - 302. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. E. Koering, G. Fourel, E. Binet-Brasselet, T. Laroche, F. Klein, and E. Gilson Identification of high affinity Tbf1p-binding sites within the budding yeast genome Nucleic Acids Res., July 1, 2000; 28(13): 2519 - 2526. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Smogorzewska, B. van Steensel, A. Bianchi, S. Oelmann, M. R. Schaefer, G. Schnapp, and T. de Lange Control of Human Telomere Length by TRF1 and TRF2 Mol. Cell. Biol., March 1, 2000; 20(5): 1659 - 1668. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. T. Kirkpatrick, Q. Fan, and T. D. Petes Maximal Stimulation of Meiotic Recombination by a Yeast Transcription Factor Requires the Transcription Activation Domain and a DNA-Binding Domain Genetics, May 1, 1999; 152(1): 101 - 115. [Abstract] [Full Text]
|
|
|
|
 |
|
 J.-H. Sohn, E.-S. Choi, H. A. Kang, J.-S. Rhee, and S.-K. Rhee A Family of Telomere-Associated Autonomously Replicating Sequences and Their Functions in Targeted Recombination in Hansenula polymorpha DL-1 J. Bacteriol., February 1, 1999; 181(3): 1005 - 1013. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. E. Eid and B. Sollner-Webb ST-2, a Telomere and Subtelomere Duplex and G-strand Binding Protein Activity in Trypanosoma brucei J. Biol. Chem., June 6, 1997; 272(23): 14927 - 14936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Marcand, E. Gilson, and D. Shore A Protein-Counting Mechanism for Telomere Length Regulation in Yeast Science, February 14, 1997; 275(5302): 986 - 990. [Abstract] [Full Text]
|
|
|
|
|
|
S Marcand, S W Buck, P Moretti, E Gilson, and D Shore Silencing of genes at nontelomeric sites in yeast is controlled by sequestration of silencing factors at telomeres by Rap 1 protein. Genes & Dev., June 1, 1996; 10(11): 1297 - 1309. [Abstract] [PDF]
|
|