Skip Navigation

This Article
Right arrow Print PDF (3508K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (43)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Semerad, C. L.
Right arrow Articles by James Maher, L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Semerad, C. L.
Right arrow Articles by James Maher, L., III
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1994, Vol. 22, No. 24 5321-5325
© 1994

Articles

Exclusion of RNA strands from a purine motif triple helix

Craig L. Semerad and L. James Maher, III*

Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Centei 600 S. 42nd Street Omaha, NE 68198-6805, USA

*To whom correspondence should be addressed

Received August 30, 1994. Accepted October 19, 1994.

Research concerning oligonucleotide-directed triple helix formation has mainly focused on the binding of DNA oligonucleotides to duplex DNA. The participation of RNA strands In triple helices is also of interest. For the pyrimldine motif (pyrlmldlne- purine (pyrimldlne triplets), systematic substitution of RNA for DNA in one, two, or all three triplex strands has previously been reported. For the purine motif (purine-purine-pyrimldlne triplets), studies have shown only that RNA cannot bind to duplex DNA. To extend this result, we created a DNA triple helix in the purine motif and systematically replaced one, two, or all three strands with RNA. In dramatic contrast to the general accommodation of RNA strands in the pyrimidine triple helix motif, a stable triplex forms In the purine motif only when all three of the substltuent strands are DNA. The lack of triplex formation among any of the other seven possible strand combinations involving RNA suggests that: (i) duplex structures containing RNA cannot be targeted by DNA oligonucleotides In the purine motif; (ii) RNA strands cannot be employed to recognize duplex DNA in the purine motif; and (ill) RNA tertiary structures are likely to contain only isolated base triplets in the purine motif.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
E. Ayel and C. Escude
In vitro selection of oligonucleotides that bind double-stranded DNA in the presence of triplex-stabilizing agents
Nucleic Acids Res., December 8, 2009; (2009) gkp1139v1.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A. Dallas, S. V. Balatskaya, T.-C. Kuo, H. Ilves, A. V. Vlassov, R. L. Kaspar, K. O. Kisich, S. A. Kazakov, and B. H. Johnston
Hairpin ribozyme-antisense RNA constructs can act as molecular lassos
Nucleic Acids Res., December 1, 2008; 36(21): 6752 - 6766.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
V. N. Potaman, E. A. Oussatcheva, Y. L. Lyubchenko, L. S. Shlyakhtenko, S. I. Bidichandani, T. Ashizawa, and R. R. Sinden
Length-dependent structure formation in Friedreich ataxia (GAA)n{middle dot}(TTC)n repeats at neutral pH
Nucleic Acids Res., February 20, 2004; 32(3): 1224 - 1231.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
S. Ivanov, Y. Alekseev, J.-R. Bertrand, C. Malvy, and M. B. Gottikh
Formation of stable triplexes between purine RNA and pyrimidine oligodeoxyxylonucleotides
Nucleic Acids Res., July 15, 2003; 31(14): 4256 - 4263.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Carmona and M. Molina
Binding of oligonucleotides to a viral hairpin forming RNA triplexes with parallel G*G*C triplets
Nucleic Acids Res., March 15, 2002; 30(6): 1333 - 1337.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
H. J. Datta and P. M. Glazer
Intracellular generation of single-stranded DNA for chromosomal triplex formation and induced recombination
Nucleic Acids Res., December 15, 2001; 29(24): 5140 - 5147.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. R. Hoyne, A. M. Gacy, C. T. McMurray, and L. J. Maher III
Stabilities of intrastrand pyrimidine motif DNA and RNA triple helices
Nucleic Acids Res., February 1, 2000; 28(3): 770 - 775.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Pascolo and J.-J. Toulme
Double Hairpin Complexes Allow Accommodation of All Four Base Pairs in Triple Helices Containing Both DNA and RNA Strands
J. Biol. Chem., September 27, 1996; 271(39): 24187 - 24192.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.