Nucleic Acids Research, 1994, Vol. 22, No. 25 5548-5554
© 1994
Articles |
DNA binding activities of the Caenorhabditis elegans Tc3 transposase
The Netherlands Cancer Institute, Division of Molecular Biology Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
*To whom correspondence should be addressed
+ Prescnt address: University of Oxford, Unit of Microbiology, Department of Biochemistry, Soudi Parks Road, Oxford OX1 3QU, UK
Received October 18, 1994. Revised November 18, 1994. Accepted November 18, 1994.
Tc3 Is a member of the Tc1/mariner family of transposable elements. All these elements have terminal Inverted repeats, encode related transposases and Insert exclusively into TA dinucleotides. We have studied the DNA binding properties of Tc3 transposase and found that an N-termlnal domain of 65 amino acids binds specifically to two regions within the 462 bp Tc3 inverted repeat; one region is located at the end of the inverted repeat, the other is located 
180 bp from the end. Methylatlon interference experiments indicate that this N-termlnal DNA binding domain of the Tc3 transposase Interacts with nucleotides on one face of the DNA helix over adjacent major and minor grooves.
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