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Nucleic Acids Research, 1994, Vol. 22, No. 25 5565-5570
© 1994

Articles

Radiolytic signature of Z-DNA

Laurence Tartier, Veslav Michalik+,, Melanie Spotheim-Maurizot, A.Rachid Rahmouni, Roland Sabattier1 and Michel Charlier*,

Centre de Biophysique Moleéculaire, CNRS rue Charles-Sadron, 45071 Orleéans Cedex 2 1Centre Hospitalier Reégional d'Orleéans, Service de Radiotheérapie 45067 Orleéans Cedex 2, France

*To whom correspondence should be addressed

+Present address: Nuclear Physics Institute, Radiation Dosimetry Department, Na Truhlarce 39, Praha 8, 18086 Czech Republic

Received October 13, 1994. Revised November 10, 1994. Accepted November 10, 1994.

Ionizing radiations induce various damages in DNA via the hydroxyl radical OH generated by the radlolysis of water. We compare here the radlosensltlvlty of B- and Z-DNA, by using a Z-prone stretch included In a plasmid. In the supercolled plasmid, the stretch Is In the Z-form, whereas it is in the B-form when the plasmid is relaxed. Frank strand breaks (FSB) and alkali-revealed breaks (ARB) were located and quantified using sequencing gel electrophoresls. We show that B- and Z-DNA have the same mean sensitivity towards radiolytic attack, for both FSB and ARB. Nevertheless, the guanlne sites are more sensitive, and the cytoslne sites less sensitive in Z- than in B-DNA, leading to a characteristic signature of the Z-form. The comparison of experiments with the outcome of a Monte Carlo simulation of OH' radical attack suggests that transfer of initial damage from a guanine base to its attached sugar or the adjacent 3' cytoslne is more Important in Z-DNA than In B-DNA.


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